Huh Jeong-Eun, Yang Ha-Ru, Park Dong-Suk, Choi Do-Young, Baek Yong-Hyeon, Cho Eun-Mi, Cho Yoon-Je, Kang-Il Kim, Kim Deog-Yoon, Lee Jae-Dong
Oriental Medicine Research Center for Bone & Joint Disease, Kyung Hee University, 1 Hoegidong, Dongdaemungu, Seoul 130-701, Republic of Korea.
J Ethnopharmacol. 2006 Apr 6;104(3):345-50. doi: 10.1016/j.jep.2005.09.041. Epub 2006 Feb 7.
Puerariae radix (PR) is a traditional medicine herb used for enhancing body resistance against various diseases. The aim of this study was to identify whether Puerariae radix extract induces osteogenic activity in human osteoblast-like SaOS-2 cells. Puerariae radix had no effect on the viability of osteoblastic cells, and dose-dependently increased alkaline phosphatase (ALP) activity. Puerariae radix markedly increased mRNA expression for vascular endothelial growth factor (VEGF), osteocalcin (OCN), osteopontin (OPN), and type I collagen (Col I) in SaOS-2 cells. Extracellular accumulation of proteins such as VEGF and Col I was increased in a dose-dependent manner. Also, Puerariae radix significantly induced mineralization in the culture of SaOS-2 cells. In conclusion, this study showed that Puerariae radix had no effect on viability, but enhanced ALP activity, VEGF, bone matrix proteins such as OCN, OPN, and Col I, and mineralization in SaOS-2 cells. These results propose that Puerariae radix can play an important role in osteoblastic bone formation, and may possibly lead to the development of bone-forming drugs.
葛根是一种用于增强机体对各种疾病抵抗力的传统草药。本研究的目的是确定葛根提取物是否能诱导人成骨样SaOS-2细胞的成骨活性。葛根对成骨细胞的活力没有影响，但能剂量依赖性地增加碱性磷酸酶（ALP）活性。葛根显著增加了SaOS-2细胞中血管内皮生长因子（VEGF）、骨钙素（OCN）、骨桥蛋白（OPN）和I型胶原（Col I）的mRNA表达。VEGF和Col I等蛋白质的细胞外积累呈剂量依赖性增加。此外，葛根在SaOS-2细胞培养中显著诱导矿化。总之，本研究表明葛根对活力没有影响，但能增强ALP活性、VEGF、骨基质蛋白如OCN、OPN和Col I以及SaOS-2细胞中的矿化。这些结果表明葛根在成骨细胞骨形成中可能发挥重要作用，并可能导致骨形成药物的开发。
