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肝脏葡萄糖-6-磷酸酶的活性不受生理性细胞内钙离子浓度的调节。

Liver glucose-6-phosphatase activity is not modulated by physiological intracellular Ca2+ concentrations.

作者信息

Fulceri R, Bellomo G, Gamberucci A, Benedetti A

机构信息

Istituto di Patologia Generale, University of Siena, Italy.

出版信息

Biochem J. 1991 May 1;275 ( Pt 3)(Pt 3):805-7. doi: 10.1042/bj2750805.

DOI:10.1042/bj2750805
PMID:1645522
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1150127/
Abstract
  1. In the presence of MgATP and increasing amounts of added Ca2+, isolated liver microsomal vesicles accumulate approx. 10 nmol of Ca2+/mg of protein and buffer ambient free Ca2+ at increasing concentrations (0.22-10.9 microM). Under these experimental conditions, microsomal glucose-6-phosphatase activity is unaffected by the concentration of extravesicular free Ca2+. 2. Different levels of intravesicular Ca2+ were obtained by treating microsomes with the Ca2+ ionophore A23187 and by stimulating active microsomal Ca2+ accumulation with Pi (3 mM). In both instances, microsomal glucose-6-phosphatase activity is unaffected by the level of intravesicular Ca2+.
摘要
  1. 在存在MgATP以及添加的Ca2+量不断增加的情况下，分离出的肝微粒体囊泡积累约10 nmol Ca2+/mg蛋白质，并在不断增加的浓度（0.22 - 10.9 microM）下缓冲周围的游离Ca2+。在这些实验条件下，微粒体葡萄糖-6-磷酸酶活性不受囊泡外游离Ca2+浓度的影响。2. 通过用Ca2+离子载体A23187处理微粒体以及用Pi（3 mM）刺激微粒体Ca2+的主动积累，可获得不同水平的囊泡内Ca2+。在这两种情况下，微粒体葡萄糖-6-磷酸酶活性均不受囊泡内Ca2+水平的影响。

相似文献

1
Liver glucose-6-phosphatase activity is not modulated by physiological intracellular Ca2+ concentrations.肝脏葡萄糖-6-磷酸酶的活性不受生理性细胞内钙离子浓度的调节。
Biochem J. 1991 May 1;275 ( Pt 3)(Pt 3):805-7. doi: 10.1042/bj2750805.
2
[Functional coupling of the calcium pump and glucose 6-phosphatase activity in liver microsomes: preliminary results].
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Calcium sequestration activity in rat liver microsomes. Evidence for a cooperation of calcium transport with glucose-6-phosphatase.大鼠肝脏微粒体中的钙螯合活性。钙转运与葡萄糖-6-磷酸酶协同作用的证据。
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Physiological concentrations of inorganic phosphate affect MgATP-dependent Ca2+ storage and inositol trisphosphate-induced Ca2+ efflux in microsomal vesicles from non-hepatic cells.无机磷酸盐的生理浓度会影响非肝细胞微粒体囊泡中MgATP依赖的Ca2+储存以及肌醇三磷酸诱导的Ca2+外流。
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Mechanism of the ATP-dependent phosphatidylserine synthesis in liver subcellular fractions.
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引用本文的文献

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Intraluminal calcium of the liver endoplasmic reticulum stimulates the glucuronidation of p-nitrophenol.肝内质网腔内的钙会刺激对硝基苯酚的葡萄糖醛酸化。
Biochem J. 1993 May 15;292 ( Pt 1)(Pt 1):99-104. doi: 10.1042/bj2920099.
2
Permeability of rat liver microsomal membrane to glucose 6-phosphate.大鼠肝微粒体膜对6-磷酸葡萄糖的通透性
Biochem J. 1992 Sep 15;286 ( Pt 3)(Pt 3):813-7. doi: 10.1042/bj2860813.

本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Ca2+-selective electrodes: a novel PVC-gelled neutral carrier mixture compared with other currently available sensors.钙离子选择性电极：一种新型聚氯乙烯凝胶化中性载体混合物与其他现有传感器的比较。
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Analysis of microsomal calcium sequestration by steady state isotope exchange. Enzyme kinetics and role of membrane permeability.
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Guanosine 5'-triphosphate releases calcium from rat liver and guinea pig parotid gland endoplasmic reticulum independently of inositol 1,4,5-trisphosphate.
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Intracellular calcium homeostasis.细胞内钙稳态
Annu Rev Biochem. 1987;56:395-433. doi: 10.1146/annurev.bi.56.070187.002143.
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Antagonistic regulation of the glucose/glucose 6-phosphate cycle by insulin and glucagon in cultured hepatocytes.胰岛素和胰高血糖素对培养肝细胞中葡萄糖/6-磷酸葡萄糖循环的拮抗调节作用。
Biochem J. 1986 Aug 15;238(1):185-91. doi: 10.1042/bj2380185.
7
Calcium sequestration activity in rat liver microsomes. Evidence for a cooperation of calcium transport with glucose-6-phosphatase.大鼠肝脏微粒体中的钙螯合活性。钙转运与葡萄糖-6-磷酸酶协同作用的证据。
Biochim Biophys Acta. 1985 Jun 27;816(2):267-77. doi: 10.1016/0005-2736(85)90494-8.
8
Effects of Ca2+ and V5+ on glucose-6-phosphatase activity in rat liver microsomes: the Ca2+ effect is reversed by regucalcin.钙离子和钒离子对大鼠肝脏微粒体中葡萄糖-6-磷酸酶活性的影响：雷古菌素可逆转钙离子的作用。
Chem Pharm Bull (Tokyo). 1989 Feb;37(2):388-90. doi: 10.1248/cpb.37.388.
9
Liver glucose-6-phosphatase activity is modulated by physiological intracellular Ca2+ concentrations.
J Biol Chem. 1989 Apr 15;264(11):6033-6.
10
MgATP-dependent accumulation of calcium ions and inorganic phosphate in a liver reticular pool.镁三磷酸腺苷依赖的钙离子和无机磷酸盐在肝脏网状池中的积聚。
Biochem J. 1990 Dec 1;272(2):549-52. doi: 10.1042/bj2720549.