肝脏葡萄糖-6-磷酸酶的活性不受生理性细胞内钙离子浓度的调节。
Liver glucose-6-phosphatase activity is not modulated by physiological intracellular Ca2+ concentrations.
作者信息
Fulceri R, Bellomo G, Gamberucci A, Benedetti A
机构信息
Istituto di Patologia Generale, University of Siena, Italy.
出版信息
Biochem J. 1991 May 1;275 ( Pt 3)(Pt 3):805-7. doi: 10.1042/bj2750805.
Abstract
- In the presence of MgATP and increasing amounts of added Ca2+, isolated liver microsomal vesicles accumulate approx. 10 nmol of Ca2+/mg of protein and buffer ambient free Ca2+ at increasing concentrations (0.22-10.9 microM). Under these experimental conditions, microsomal glucose-6-phosphatase activity is unaffected by the concentration of extravesicular free Ca2+. 2. Different levels of intravesicular Ca2+ were obtained by treating microsomes with the Ca2+ ionophore A23187 and by stimulating active microsomal Ca2+ accumulation with Pi (3 mM). In both instances, microsomal glucose-6-phosphatase activity is unaffected by the level of intravesicular Ca2+.
摘要
- 在存在MgATP以及添加的Ca2+量不断增加的情况下，分离出的肝微粒体囊泡积累约10 nmol Ca2+/mg蛋白质，并在不断增加的浓度（0.22 - 10.9 microM）下缓冲周围的游离Ca2+。在这些实验条件下，微粒体葡萄糖-6-磷酸酶活性不受囊泡外游离Ca2+浓度的影响。2. 通过用Ca2+离子载体A23187处理微粒体以及用Pi（3 mM）刺激微粒体Ca2+的主动积累，可获得不同水平的囊泡内Ca2+。在这两种情况下，微粒体葡萄糖-6-磷酸酶活性均不受囊泡内Ca2+水平的影响。
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