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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Fulceri R, Bellomo G, Gamberucci A, Benedetti A

Istituto di Patologia Generale, University of Siena, Italy.

Biochem J. 1990 Dec 1;272(2):549-52. doi: 10.1042/bj2720549.

MgATP-dependent Ca2+ uptake by rat liver microsomal preparations and permeabilized hepatocytes was measured in the presence or absence of Pi. 2. Monitoring of free Ca2+ in incubation systems with a Ca2+ electrode in the presence of Pi (2-7 mM) revealed a biphasic Ca2+ uptake, with the onset of a second, Pi-dependent, Ca2+ accumulation. 3. Increasing Pi concentrations (up to 10 mM) caused a progressive enlargement of 45Ca2(+)-loading capacity of microsomal fractions. 4. As a result of Pi stimulation of active Ca2+ uptake, [32P]Pi and 45Ca2+ were co-accumulated. 5. Experiments with permeabilized hepatocytes revealed that the amount of Ca2+ releasable by myo-inositol 1,4,5-trisphosphate is unaffected by Pi.

在有或无无机磷酸盐（Pi）存在的情况下，测定了大鼠肝脏微粒体制剂和透化肝细胞对MgATP依赖性的Ca2+摄取。2. 在存在Pi（2 - 7 mM）的情况下，用Ca2+电极监测孵育系统中的游离Ca2+，发现Ca2+摄取呈双相性，出现了第二个依赖于Pi的Ca2+积累阶段。3. 增加Pi浓度（高达10 mM）导致微粒体组分的45Ca2(+)-负载能力逐渐增大。4. 由于Pi刺激了活性Ca2+摄取，[32P]Pi和45Ca2+共同积累。5. 对透化肝细胞进行的实验表明，肌醇1,4,5-三磷酸可释放的Ca2+量不受Pi的影响。

Fulceri R, Bellomo G, Gamberucci A, Benedetti A

Istituto di Patologia Generale, University of Siena, Italy.

Biochem J. 1990 Dec 1;272(2):549-52. doi: 10.1042/bj2720549.

MgATP-dependent Ca2+ uptake by rat liver microsomal preparations and permeabilized hepatocytes was measured in the presence or absence of Pi. 2. Monitoring of free Ca2+ in incubation systems with a Ca2+ electrode in the presence of Pi (2-7 mM) revealed a biphasic Ca2+ uptake, with the onset of a second, Pi-dependent, Ca2+ accumulation. 3. Increasing Pi concentrations (up to 10 mM) caused a progressive enlargement of 45Ca2(+)-loading capacity of microsomal fractions. 4. As a result of Pi stimulation of active Ca2+ uptake, [32P]Pi and 45Ca2+ were co-accumulated. 5. Experiments with permeabilized hepatocytes revealed that the amount of Ca2+ releasable by myo-inositol 1,4,5-trisphosphate is unaffected by Pi.

在有或无无机磷酸盐（Pi）存在的情况下，测定了大鼠肝脏微粒体制剂和透化肝细胞对MgATP依赖性的Ca2+摄取。2. 在存在Pi（2 - 7 mM）的情况下，用Ca2+电极监测孵育系统中的游离Ca2+，发现Ca2+摄取呈双相性，出现了第二个依赖于Pi的Ca2+积累阶段。3. 增加Pi浓度（高达10 mM）导致微粒体组分的45Ca2(+)-负载能力逐渐增大。4. 由于Pi刺激了活性Ca2+摄取，[32P]Pi和45Ca2+共同积累。5. 对透化肝细胞进行的实验表明，肌醇1,4,5-三磷酸可释放的Ca2+量不受Pi的影响。