Hay F G, Duncan L W, Langdon S P, Leonard R C
Imperial Cancer Research Fund Medical Oncology Unit, Western General Hospital, Edinburgh, UK.
Br J Cancer Suppl. 1991 Jun;14:33-5.
The expression of the human small cell lung cancer (SCLC) cluster 1 antigen and the human milk fat globule membrane (HMFG) antigen were studied in three SCLC, three lung adenocarcinoma, six ovarian adenocarcinoma and three colorectal adenocarcinoma cell lines before and after culture in the presence of the differentiation inducing agent, sodium butyrate. Before treatment, only SCLC and well differentiated ovarian cell lines expressed the cluster 1 antigen. After 4 days culture with sodium butyrate, expression of cluster 1 antigen was induced in poorly differentiated ovarian, colorectal and lung adenocarcinoma cell lines whereas existing antigen expression was enhanced in SCLC and well differentiated ovarian cell lines. The expression of the HMFG antigen was also modified. Induction of cluster 1 antigen correlated with increased levels of alkaline phosphatase in the treated cell lines, this enzyme being associated with a more differentiated state in colon and ovarian carcinoma cell lines. The pattern of induction of cluster 1 antigen expression suggests that several different epitopes of this antigen are recognised by the ten SCLC cluster 1 antibodies studied.
在分化诱导剂丁酸钠存在的情况下，对三种小细胞肺癌（SCLC）、三种肺腺癌、六种卵巢腺癌和三种结肠腺癌细胞系进行培养前后，研究了人小细胞肺癌1簇抗原和人乳脂肪球膜（HMFG）抗原的表达。治疗前，只有SCLC和高分化卵巢细胞系表达1簇抗原。用丁酸钠培养4天后，低分化卵巢、结肠和肺腺癌细胞系中诱导了1簇抗原的表达，而SCLC和高分化卵巢细胞系中现有的抗原表达增强。HMFG抗原的表达也发生了改变。1簇抗原的诱导与处理后的细胞系中碱性磷酸酶水平的增加相关，这种酶与结肠和卵巢癌细胞系中更分化的状态有关。1簇抗原表达的诱导模式表明，所研究的十种SCLC 1簇抗体识别该抗原的几种不同表位。
