Niimura M, Pass F, Wooley R, Soutor C A
J Natl Cancer Inst. 1975 Mar;54(3):563-9.
The formation of human skin warts may depend on transformation by human papilloma virus or result from a productive virus infection. To explore this problem, methods were developed for the primary tissue culture of epidermal cells (keratinocytes) derived from wart tissues and normal skin. Wart-derived keratinocytes continued to proliferate for periods exceeding 3 months, but the patterns of cell growth were identical to normal keratinocytes. Electron microscopic and immunohistologic examination of wart-derived cultures did not evidence virion assembly.
人类皮肤疣的形成可能取决于人乳头瘤病毒的转化作用，或者源于病毒的增殖性感染。为了探究这个问题，人们开发了一些方法，用于对源自疣组织和正常皮肤的表皮细胞（角质形成细胞）进行原代组织培养。源自疣组织的角质形成细胞持续增殖超过3个月，但细胞生长模式与正常角质形成细胞相同。对源自疣组织的培养物进行电子显微镜和免疫组织学检查，未发现病毒粒子装配的迹象。
