Tagliabue E, Martignone S, Mastroianni A, Ménard S, Pellegrini R, Colnaghi M I
Division of Experimental Oncology E, Istituto Nazionale Tumori, Milan, Italy.
Br J Cancer Suppl. 1991 Jun;14:83-5.
Several studies have suggested a correlation between the metastatic potential and the expression of adhesion molecules and/or their receptors on tumour cell surface membranes. In this study we investigated the expression of functional laminin receptors on small cell lung cancer (SCLC) cells. To this aim we set up an adherence assay to determine the in vitro binding capability of tumour cell lines to laminin. All of the three SCLC lines tested and cells from a short-term SCLC line adhered to laminin in cell culture plates, and the affinity of cell-matrix adhesion proved to be higher than the cell-cell adhesion. This effect was always laminin dose-dependent. On a laminin affinity chromatography column three major proteins could be eluted with EDTA from soluble extracts of SCLC lines. Their molecular weights of 120, 90 and 30 kDa suggested a possible relationship with the integrin family. This putative integrin laminin receptor expressed on SCLC does not react with fibronectin, vitronectin or collagen.
多项研究表明，肿瘤细胞表面膜上粘附分子及其受体的表达与转移潜能之间存在关联。在本研究中，我们调查了小细胞肺癌（SCLC）细胞上功能性层粘连蛋白受体的表达。为此，我们建立了一种粘附试验，以确定肿瘤细胞系与层粘连蛋白的体外结合能力。所测试的所有三个SCLC系以及来自一个短期SCLC系的细胞均在细胞培养板中粘附于层粘连蛋白，并且细胞与基质的粘附亲和力被证明高于细胞与细胞之间的粘附。这种效应始终呈层粘连蛋白剂量依赖性。在层粘连蛋白亲和层析柱上，可以用EDTA从SCLC系的可溶性提取物中洗脱三种主要蛋白质。它们120、90和30 kDa的分子量表明可能与整合素家族有关。这种在SCLC上表达的假定整合素层粘连蛋白受体不与纤连蛋白、玻连蛋白或胶原蛋白发生反应。
