Martignone S, Pellegrini R, Villa E, Tandon N N, Mastroianni A, Tagliabue E, Ménard S, Colnaghi M I
Istituto Nazionale Tumori, Milan, Italy.
Clin Exp Metastasis. 1992 Nov;10(6):379-86. doi: 10.1007/BF00133466.
Two monoclonal antibodies (mAbs), designated MLuC5 and MLuC6, were produced against a human small cell lung carcinoma cell line. They were found to exhibit a superimposable reactivity on different cell lines and on platelets. Moreover, they both immunoprecipitated a 67 kDa molecule from the membrane of the reference target cells. Immunodepletion and cross-inhibition tests indicated that the two mAbs recognize two epitopes closely localized on the same molecule. The MLuC5 mAb was further characterized for its reactivity on platelets. Immunoprecipitation and ELISA assays demonstrate that this mAb recognizes the 67 kDa high affinity laminin receptor. MLuC5 reactivity was evaluated by immunohistochemistry on a variety of normal and tumor tissues, in particular breast specimens including normal epithelium, dysplastic lesions, in situ carcinomas, invasive primary carcinomas and distant metastases. The laminin receptor was found to be strongly expressed in 50% of the infiltrating carcinomas, whereas in situ carcinomas and benign lesions, as well as the normal mammary epithelium, were only weakly and focally positive. In metastatic lesions MLuC5 reactivity was only found in 11% of the samples tested, independently of the site of origin of the lesion.
制备了两种针对人小细胞肺癌细胞系的单克隆抗体（mAb），分别命名为MLuC5和MLuC6。发现它们在不同细胞系和血小板上表现出重叠的反应性。此外，它们都从参考靶细胞的膜上免疫沉淀出一个67 kDa的分子。免疫去除和交叉抑制试验表明，这两种单克隆抗体识别紧密定位在同一分子上的两个表位。进一步对MLuC5单克隆抗体在血小板上的反应性进行了表征。免疫沉淀和ELISA分析表明，该单克隆抗体识别67 kDa的高亲和力层粘连蛋白受体。通过免疫组织化学对多种正常和肿瘤组织，特别是乳腺标本，包括正常上皮、发育异常病变、原位癌、浸润性原发性癌和远处转移灶，评估了MLuC5的反应性。发现层粘连蛋白受体在50%的浸润性癌中强烈表达，而原位癌和良性病变以及正常乳腺上皮仅呈弱阳性且局灶阳性。在转移灶中，仅在11%的测试样本中发现MLuC5反应性，与病变的起源部位无关。
