Russo Cristina, Tortoli Enrico, Menichella Donato
Centro Regionale di Riferimento per la Diagnostica dei Micobatteri, Piastra dei Servizi, Ospedale di Careggi, viale Morgagni 85, 50134 Firenze, Italy.
J Clin Microbiol. 2006 Feb;44(2):334-9. doi: 10.1128/JCM.44.2.334-339.2006.
The new commercial assay GenoType Mycobacterium, intended for the identification of mycobacteria, was evaluated with a panel of 197 strains belonging to 86 different taxa. The system, which relies on solid-phase reverse hybridization, is composed of two different kits: GenoType CM for the identification of more frequently detected mycobacterial species and GenoType AS for less common species. The sensitivity and specificity were 97.9% and 92.4% for the CM kit and 99.3% and 99.4% for the AS kit. Our results show that the system may represent a useful tool to substantially enlarge the number of mycobacteria that can be reliably identified in clinical laboratories.
新型商业检测方法GenoType Mycobacterium旨在鉴定分枝杆菌,我们使用一组属于86个不同分类单元的197株菌株对其进行了评估。该系统依靠固相反向杂交技术,由两种不同的试剂盒组成:用于鉴定更常见分枝杆菌种类的GenoType CM试剂盒和用于鉴定较不常见种类的GenoType AS试剂盒。CM试剂盒的灵敏度和特异性分别为97.9%和92.4%,AS试剂盒的灵敏度和特异性分别为99.3%和99.4%。我们的结果表明,该系统可能是一种有用的工具,可大幅增加临床实验室能够可靠鉴定的分枝杆菌数量。