新型GenoType分枝杆菌检测法用于分枝杆菌菌种鉴定的评估。
Evaluation of the new GenoType Mycobacterium assay for identification of mycobacterial species.
作者信息
Russo Cristina, Tortoli Enrico, Menichella Donato
机构信息
Centro Regionale di Riferimento per la Diagnostica dei Micobatteri, Piastra dei Servizi, Ospedale di Careggi, viale Morgagni 85, 50134 Firenze, Italy.
出版信息
J Clin Microbiol. 2006 Feb;44(2):334-9. doi: 10.1128/JCM.44.2.334-339.2006.
Abstract
The new commercial assay GenoType Mycobacterium, intended for the identification of mycobacteria, was evaluated with a panel of 197 strains belonging to 86 different taxa. The system, which relies on solid-phase reverse hybridization, is composed of two different kits: GenoType CM for the identification of more frequently detected mycobacterial species and GenoType AS for less common species. The sensitivity and specificity were 97.9% and 92.4% for the CM kit and 99.3% and 99.4% for the AS kit. Our results show that the system may represent a useful tool to substantially enlarge the number of mycobacteria that can be reliably identified in clinical laboratories.
摘要
新型商业检测方法GenoType Mycobacterium旨在鉴定分枝杆菌，我们使用一组属于86个不同分类单元的197株菌株对其进行了评估。该系统依靠固相反向杂交技术，由两种不同的试剂盒组成：用于鉴定更常见分枝杆菌种类的GenoType CM试剂盒和用于鉴定较不常见种类的GenoType AS试剂盒。CM试剂盒的灵敏度和特异性分别为97.9%和92.4%，AS试剂盒的灵敏度和特异性分别为99.3%和99.4%。我们的结果表明，该系统可能是一种有用的工具，可大幅增加临床实验室能够可靠鉴定的分枝杆菌数量。
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