INNO-LiPA分枝杆菌v2检测法的评估：用于分枝杆菌鉴定的改进型反向杂交多重DNA探针检测法
Evaluation of INNO-LiPA MYCOBACTERIA v2: improved reverse hybridization multiple DNA probe assay for mycobacterial identification.
作者信息
Tortoli Enrico, Mariottini Alessandro, Mazzarelli Gianna
机构信息
Regional Reference Center for Mycobacteria, Microbiology and Virology Laboratory, Careggi Hospital, Florence, Italy.
出版信息
J Clin Microbiol. 2003 Sep;41(9):4418-20. doi: 10.1128/JCM.41.9.4418-4420.2003.
Abstract
INNO-LiPA MYCOBACTERIA (Innogenetics, Ghent, Belgium) is a reverse hybridization DNA probe assay that has been recently improved by increasing the number of identifiable mycobacterial species to 16. Our assessment, performed with 197 mycobacteria belonging to 81 taxa, revealed 100% specificity and sensitivity for 20 out of 23 probes. The probes specific for Mycobacterium fortuitum complex, for the Mycobacterium avium-intracellulare-scrofulaceum group, and for Mycobacterium intracellulare type 2 cross-reacted with several mycobacteria rarely isolated from clinical specimens. The overall sensitivity was 100%, and the overall specificity was 94.4%.
摘要
INNO-LiPA分枝杆菌检测法（比利时根特的Innogenetics公司）是一种反向杂交DNA探针检测法，最近通过将可鉴定的分枝杆菌种类增加到16种而得到改进。我们用属于81个分类单元的197株分枝杆菌进行的评估显示，23种探针中有20种的特异性和敏感性均为100%。针对偶然分枝杆菌复合体、鸟分枝杆菌-胞内分枝杆菌-瘰疬分枝杆菌组以及胞内分枝杆菌2型的探针与几种很少从临床标本中分离出的分枝杆菌发生交叉反应。总体敏感性为100%，总体特异性为94.4%。
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