Marcelino José Maria, Barroso Helena, Gonçalves Fátima, Silva Sofia Marques, Novo Carlos, Gomes Perpétua, Camacho Ricardo, Taveira Nuno
Unidade de Retrovírus e Infecções Associadas, Centro de Patogénese Molecular, Faculdade de Farmácia de Lisboa, Avenida das Forças Armadas, 1649-019 Lisboa, Portugal.
J Clin Microbiol. 2006 Feb;44(2):607-11. doi: 10.1128/JCM.44.2.607-611.2006.
A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.
一种针对人类免疫缺陷病毒2型(HIV-2)包膜蛋白的双抗原酶联免疫吸附测定法(ELISA-HIV2),是利用从HIV-2包膜衍生的两种新型重组多肽rpC2-C3和rgp36开发的。使用HIV-2、HIV-1和HIV-1/2样本测定了其诊断性能。两种多肽均显示出100%的特异性。rgp36的临床敏感性为100%,rpC2-C3为93.4%。ELISA-HIV2可用于HIV-2感染的特异性诊断和确认。