Gueimonde Miguel, Sakata Shinji, Kalliomäki Marko, Isolauri Erika, Benno Yoshimi, Salminen Seppo
Functional Foods Forum, University of Turku, Itäinen Pitkäkatu 4A 5th Floor, FIN-20014 Turku, Finland.
J Pediatr Gastroenterol Nutr. 2006 Feb;42(2):166-70. doi: 10.1097/01.mpg.0000189346.25172.fd.
Establishment of the gut microbiota at birth provides a substantial source of microbial stimuli for the maturation of the immune system. Deviations in this process precede the development of specific diseases providing the rationale for the use of probiotics to counteract them.
This study was designed to characterize both the mother-infant bifidobacteria transfer at birth and the development of bifidobacteria microbiota during the first weeks of life in infants whose mothers received Lactobacillus rhamnosus GG or placebo.
Species-specific PCR was used to assess the fecal bifidobacterial composition of mothers before and after delivery and in infants at 5 days and 3 weeks of age.
Bifidobacterium longum was the species most commonly found in the mothers. Bifidobacterium catenulatum was the most prevalent group in infants at 5 days of age and B. longum the predominant species at 3 weeks. At 5 days of age, infants whose mothers received L. rhamnosus GG showed a significantly higher occurrence of B. breve and lower of B. adolescentis than those from the placebo group. In addition, L. rhamnosus GG consumption increased the bifidobacterial diversity in infants and reduced the Bifidobacterium microbiota similarity between mother and infant.
These results indicate that specific changes in the transfer and initial establishment of bifidobacteria in neonates take place as consequence of the consumption of L. rhamnosus GG by the mothers.
出生时肠道微生物群的建立为免疫系统的成熟提供了大量的微生物刺激源。这一过程中的偏差先于特定疾病的发展，为使用益生菌来对抗这些偏差提供了理论依据。
本研究旨在描述出生时母婴双歧杆菌的转移情况以及母亲接受鼠李糖乳杆菌GG或安慰剂的婴儿在出生后最初几周双歧杆菌微生物群的发育情况。
采用种特异性PCR评估母亲分娩前后以及5日龄和3周龄婴儿粪便中双歧杆菌的组成。
长双歧杆菌是母亲中最常见的菌种。链状双歧杆菌是5日龄婴儿中最普遍的菌群，而长双歧杆菌是3周龄时的优势菌种。在5日龄时，母亲接受鼠李糖乳杆菌GG的婴儿短双歧杆菌的出现率显著高于安慰剂组婴儿，青春双歧杆菌的出现率则低于安慰剂组婴儿。此外，食用鼠李糖乳杆菌GG增加了婴儿双歧杆菌的多样性，并降低了母婴之间双歧杆菌微生物群的相似性。
这些结果表明，母亲食用鼠李糖乳杆菌GG会导致新生儿双歧杆菌转移和初始定植发生特定变化。
