Yuan Feng, Yu Guang-Rong, Chang Shi-Min, Lineaweaver William C, Zhang Feng
Department of Orthopedic Surgery, Tongji Hospital, Tongji University, Shanghai, China.
J Reconstr Microsurg. 2006 Feb;22(2):129-36. doi: 10.1055/s-2006-932508.
This study evaluated the effect of exogenous vascular endothelial growth factor (VEGF) on augmentation of collateral circulation in a rat hindlimb replantation model. Twenty-eight rats, divided into four groups, underwent right hindlimb replantation. In experimental Group 1, the rats received daily intramuscular VEGF injections (1 microg/kg). In Group 2, the rats received 25 microg/kg VEGF injections. In Group 3, the rats received 50 microg/kg VEGF injections. In Group 4, the control group, rats received saline injections. Seven days after replantation, the repaired femoral vessels were excised. Seven days after excision of the femoral vessels, toe necrosis ratios of the replanted hindlimbs were grossly examined, and calf blood pressures were measured. Angiography was performed and muscle tissue was biopsied for histology. The results showed that administration of VEGF significantly decreased toe necrosis and improved calf blood pressure ratio, angiographic score, and capillary density in the replanted hindlimbs that underwent late vessel excision. The effect of VEGF was significantly dose-dependent. The authors conclude that VEGF can augment angiogenesis and collateral vessel formation in replanted limbs, and improve survival in replanted limbs with potential late arterial insufficiency.
本研究评估了外源性血管内皮生长因子（VEGF）对大鼠后肢再植模型中侧支循环增强的影响。28只大鼠被分为四组，接受右后肢再植手术。在实验组1中，大鼠每天接受肌肉注射VEGF（1微克/千克）。在第2组中，大鼠接受25微克/千克VEGF注射。在第3组中，大鼠接受50微克/千克VEGF注射。在第4组（对照组）中，大鼠接受生理盐水注射。再植术后7天，切除修复的股血管。股血管切除7天后，大体检查再植后肢的趾坏死率，并测量小腿血压。进行血管造影，并取肌肉组织进行组织学活检。结果显示，给予VEGF可显著降低趾坏死率，并改善晚期血管切除的再植后肢的小腿血压比值、血管造影评分和毛细血管密度。VEGF的作用具有显著的剂量依赖性。作者得出结论，VEGF可增强再植肢体的血管生成和侧支血管形成，并提高有潜在晚期动脉供血不足的再植肢体的存活率。
