Kellis G, Shaffer J W, Field G
Department of Orthopaedic Surgery, Case Western Reserve University, Cleveland, OH 44106.
J Hand Surg Am. 1991 Nov;16(6):1070-3. doi: 10.1016/s0363-5023(10)80071-1.
The development of collateral circulation was studied in a replanted rat hindlimb model by use of the hemodynamic techniques of hydrogen washout and laser Doppler capillary perfusion monitoring. Collateral development occurred and provided blood flow whose magnitude increased linearly over time. Twenty-seven percent of preligation blood flow was present at 2 weeks after replantation, 39% at 4 to 5 weeks, and 77% at 8 to 9 weeks by laser Doppler assessment. Laser Doppler correlated more closely with limb survival than did the hydrogen washout technique. Uniform necrosis (eight of eight) occurred after pedicle interruption in the 2-week group. Partial limb survival was noted in five of eight replants at 4 to 5 weeks on transection of the vascular pedicle, while complete survival of all replants (nine of nine) was seen at 8 to 9 weeks.
通过氢气洗脱和激光多普勒毛细血管灌注监测等血流动力学技术，在大鼠后肢再植模型中研究了侧支循环的发育情况。侧支循环得以形成并提供血流，其流量随时间呈线性增加。根据激光多普勒评估，再植后2周时侧支循环血流量为结扎前血流量的27%，4至5周时为39%，8至9周时为77%。与氢气洗脱技术相比，激光多普勒与肢体存活的相关性更为密切。在2周组中，蒂部中断后均出现了完全坏死（8只均如此）。在4至5周时，8只再植肢体中有5只在血管蒂横断后出现部分肢体存活，而在8至9周时，所有再植肢体（9只均如此）均完全存活。
