Baek Yong Hyeon, Huh Jeong Eun, Lee Jae Dong, Choi Do Young, Park Dong Suk
Department of Acupuncture and Moxibustion, College of Oriental Medicine, Kyung Hee University, #1 Hoegidong, Dongdaemungu, Seoul 130-702, South Korea.
Brain Res. 2006 Feb 16;1073-1074:305-10. doi: 10.1016/j.brainres.2005.12.086. Epub 2006 Feb 2.
The antinociceptive effect and the mechanism of bee venom acupuncture (BVA) on inflammatory pain, especially in the rat model of collagen-induced arthritis (CIA), have not yet been fully studied. This study was designed to investigate the antinociceptive effect and its mu-opioid and alpha2-adrenergic mechanism of BVA in the CIA rat model. To induce CIA, male Sprague-Dawley rats were immunized with bovine type II collagen emulsified in Freund's incomplete adjuvant followed by a booster injection 14 days later. The antinociceptive effect was evaluated by tail flick latency (TFL). After induction of arthritis, the inflammatory pain threshold decreased as time passed, and there was no big change of the pain threshold after 3 weeks. Three weeks after the first immunization, BVA (0.25 mg/kg) injected into the Zusanli acupoint (ST36) showed the antinociceptive effect. Furthermore, the antinociceptive effect of BVA was blocked by yohimbine (alpha2-adrenergic receptor antagonist, 2 mg/kg, i.p) pretreatment, but not by naloxone (mu-opioid receptor antagonist, 2 mg/kg, i.p.) pretreatment. These results suggest that BVA can relieve inflammatory pain in CIA and the antinociceptive effect of BVA can be mediated by alpha2-adrenergic receptor.
蜂毒针刺疗法（BVA）对炎性疼痛，尤其是胶原诱导性关节炎（CIA）大鼠模型炎性疼痛的镇痛作用及其机制尚未得到充分研究。本研究旨在探讨BVA在CIA大鼠模型中的镇痛作用及其μ-阿片受体和α2-肾上腺素能机制。为诱导CIA，将雄性Sprague-Dawley大鼠用弗氏不完全佐剂乳化的牛II型胶原免疫，14天后进行加强注射。通过甩尾潜伏期（TFL）评估镇痛效果。关节炎诱导后，炎性疼痛阈值随时间降低，3周后疼痛阈值无明显变化。首次免疫3周后，将BVA（0.25mg/kg）注射到足三里穴（ST36）显示出镇痛作用。此外，BVA的镇痛作用被育亨宾（α2-肾上腺素能受体拮抗剂，2mg/kg，腹腔注射）预处理阻断，但未被纳洛酮（μ-阿片受体拮抗剂，2mg/kg，腹腔注射）预处理阻断。这些结果表明，BVA可缓解CIA中的炎性疼痛，且BVA的镇痛作用可由α2-肾上腺素能受体介导。
