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猴病毒40（SV40）T抗原通过SV40晚期启动子的Oct/SPH区域介导转录激活。

Simian virus 40 (SV40) T-antigen transcriptional activation mediated through the Oct/SPH region of the SV40 late promoter.

作者信息

Gruda M C, Alwine J C

机构信息

Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia 19104-6148.

出版信息

J Virol. 1991 Jul;65(7):3553-8. doi: 10.1128/JVI.65.7.3553-3558.1991.

DOI:10.1128/JVI.65.7.3553-3558.1991
PMID:1645783
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC241352/
Abstract

Simian virus 40 (SV40) large T antigen is a promiscuous transcriptional activator of many viral and cellular promoters. The SV40 late promoter, a primary target for T-antigen transcriptional activation, contains a previously described T-antigen-activatable binding site (SV40 nucleotides 186 to 225). The T-antigen-activatable binding site element contains overlapping octamer (Oct)- and SPH (TEF-1)-binding sites (Oct/SPH site). Using this Oct/SPH site as an upstream element in a simple promoter, we show that the SPH sites are necessary for transcriptional activation by T antigen. In addition, we show that when Oct 1 is overproduced, it can eliminate T-antigen-mediated transcriptional activation, as well as basal activity, from the simple Oct/SPH promoter as well as the intact SV40 late promoter. This suggests that one function of T antigen in transcriptional activation of the late promoter is to alter factor binding at the Oct/SPH region to favor binding of factors to the SPH sites.

摘要

猴病毒40（SV40）大T抗原是许多病毒和细胞启动子的一种多效转录激活因子。SV40晚期启动子是T抗原转录激活的主要靶点，它含有一个先前描述的T抗原可激活结合位点（SV40核苷酸186至225）。T抗原可激活结合位点元件包含重叠的八聚体（Oct）和SPH（TEF-1）结合位点（Oct/SPH位点）。在一个简单启动子中使用这个Oct/SPH位点作为上游元件，我们发现SPH位点对于T抗原的转录激活是必需的。此外，我们还发现，当Oct 1过量表达时，它可以消除T抗原介导的转录激活以及来自简单Oct/SPH启动子和完整SV40晚期启动子的基础活性。这表明T抗原在晚期启动子转录激活中的一个功能是改变Oct/SPH区域的因子结合，以利于因子与SPH位点的结合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5963/241352/8f69660c4b39/jvirol00050-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5963/241352/5ea095e5d4ef/jvirol00050-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5963/241352/8f69660c4b39/jvirol00050-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5963/241352/5ea095e5d4ef/jvirol00050-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5963/241352/8f69660c4b39/jvirol00050-0152-a.jpg

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本文引用的文献

1
Site-specific base substitution and deletion mutations that enhance or suppress transcription of the SV40 major late RNA.增强或抑制SV40主要晚期RNA转录的位点特异性碱基置换和缺失突变。
Cell. 1982 Dec;31(3 Pt 2):625-33. doi: 10.1016/0092-8674(82)90318-x.
2
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
Mol Cell Biol. 1982 Sep;2(9):1044-51. doi: 10.1128/mcb.2.9.1044-1051.1982.
3
Activation of the SV40 late promoter: direct effects of T antigen in the absence of viral DNA replication.
大T抗原对JC病毒早期启动子的胶质细胞特异性调控。
J Virol. 2000 Jan;74(2):755-63. doi: 10.1128/jvi.74.2.755-763.2000.
4
A kinase activity associated with simian virus 40 large T antigen phosphorylates upstream binding factor (UBF) and promotes formation of a stable initiation complex between UBF and SL1.与猿猴病毒40大T抗原相关的激酶活性使上游结合因子（UBF）磷酸化，并促进UBF与SL1之间稳定起始复合物的形成。
Mol Cell Biol. 1999 Apr;19(4):2791-802. doi: 10.1128/MCB.19.4.2791.
5
Simian virus 40 large T antigen stabilizes the TATA-binding protein-TFIIA complex on the TATA element.猿猴病毒40大T抗原可使TATA元件上的TATA结合蛋白-TFIIA复合物稳定。
Mol Cell Biol. 1998 Jul;18(7):3926-35. doi: 10.1128/MCB.18.7.3926.
6
Simian virus 40 large T antigen interacts with human TFIIB-related factor and small nuclear RNA-activating protein complex for transcriptional activation of TATA-containing polymerase III promoters.猿猴病毒40大T抗原与人类TFIIB相关因子及小核RNA激活蛋白复合体相互作用，以激活含TATA的聚合酶III启动子的转录。
Mol Cell Biol. 1998 Mar;18(3):1331-8. doi: 10.1128/MCB.18.3.1331.
7
Mechanisms of transcriptional regulation of cellular genes by SV40 large T- and small T-antigens.SV40 大 T 抗原和小 T 抗原对细胞基因转录调控的机制。
Virus Genes. 1997;15(2):135-54. doi: 10.1023/a:1007962908248.
8
TAF-like functions of human cytomegalovirus immediate-early proteins.人类巨细胞病毒立即早期蛋白的TAF样功能
J Virol. 1997 Oct;71(10):7227-39. doi: 10.1128/JVI.71.10.7227-7239.1997.
9
Interaction between T antigen and TEA domain of the factor TEF-1 derepresses simian virus 40 late promoter in vitro: identification of T-antigen domains important for transcription control.T抗原与转录增强因子TEF-1的TEA结构域之间的相互作用在体外可解除猿猴病毒40晚期启动子的抑制：鉴定对转录控制重要的T抗原结构域。
J Virol. 1996 Feb;70(2):1203-12. doi: 10.1128/JVI.70.2.1203-1212.1996.
10
Negative regulatory elements upstream of a novel exon of the neuronal nicotinic acetylcholine receptor alpha 2 subunit gene.神经元烟碱型乙酰胆碱受体α2亚基基因一个新外显子上游的负调控元件。
Nucleic Acids Res. 1993 May 11;21(9):2185-92. doi: 10.1093/nar/21.9.2185.
SV40晚期启动子的激活：在无病毒DNA复制情况下T抗原的直接作用
Cell. 1984 Feb;36(2):381-9. doi: 10.1016/0092-8674(84)90231-9.
4
Deletion mapping of DNA regions required for SV40 early region promoter function in vivo.体内SV40早期区域启动子功能所需DNA区域的缺失图谱分析。
J Mol Appl Genet. 1982;1(5):457-81.
5
Late messenger RNA production by viable simian virus 40 mutants with deletions in the leader region.在先导区有缺失的存活猿猴病毒40突变体的晚期信使核糖核酸产生
J Mol Biol. 1981 Dec 15;153(3):589-618. doi: 10.1016/0022-2836(81)90409-5.
6
A paranuclear extract contains a unique set of viral transcripts late in SV40 infection.在猴病毒40（SV40）感染后期，核旁提取物包含一组独特的病毒转录本。
Virology. 1981 Sep;113(2):663-71. doi: 10.1016/0042-6822(81)90195-1.
7
High-efficiency transformation of mammalian cells by plasmid DNA.质粒DNA对哺乳动物细胞的高效转化
Mol Cell Biol. 1987 Aug;7(8):2745-52. doi: 10.1128/mcb.7.8.2745-2752.1987.
8
trans-activation of RNA polymerase II and III promoters by SV40 small t antigen.SV40小t抗原对RNA聚合酶II和III启动子的反式激活作用。
Cell. 1988 Dec 23;55(6):1171-7. doi: 10.1016/0092-8674(88)90261-9.
9
The simian virus 40 large T antigen. A lot packed into a little.猿猴病毒40大T抗原。小体积蕴含大容量。
Mol Biol Med. 1987 Apr;4(2):63-80.
10
The sequence motifs that are involved in SV40 enhancer function also control SV40 late promoter activity.参与SV40增强子功能的序列基序也控制SV40晚期启动子活性。
Nucleic Acids Res. 1987 Mar 25;15(6):2445-61. doi: 10.1093/nar/15.6.2445.