Tiley L S, Malim M H, Cullen B R
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710.
J Virol. 1991 Jul;65(7):3877-81. doi: 10.1128/JVI.65.7.3877-3881.1991.
Visna virus encodes a posttranscriptional regulatory protein that is functionally analogous to the Rev trans activator of human immunodeficiency virus type 1. Here, we demonstrate that the known functional organization of the human immunodeficiency virus type 1 Rev trans activator is shared by the distantly related visna virus Rev protein. In particular, both Rev proteins contain an N-terminal domain marked by a highly basic core motif that determines RNA sequence specificity, as well as a second C-terminal domain containing an essential leucine-rich motif that functions as an activation domain. Chimeric proteins consisting of the binding domain of one Rev protein fused to the activation domain of the other were fully functional in the viral sequence context cognate for the binding domain. We also describe derivatives of visna virus Rev bearing a defective activation domain that displayed a trans-dominant negative phenotype in transfected cells. These visna virus Rev mutants may prove useful in the derivation of transgenic animals resistant to this agriculturally important retroviral pathogen.
维斯纳病毒编码一种转录后调节蛋白，其功能类似于人类免疫缺陷病毒1型的Rev反式激活因子。在此，我们证明了人类免疫缺陷病毒1型Rev反式激活因子已知的功能组织与远亲的维斯纳病毒Rev蛋白相同。特别是，两种Rev蛋白都含有一个以高度碱性的核心基序为特征的N端结构域，该基序决定RNA序列特异性，以及第二个C端结构域，该结构域含有一个作为激活结构域的必需的富含亮氨酸基序。由一种Rev蛋白的结合结构域与另一种Rev蛋白的激活结构域融合而成的嵌合蛋白在与结合结构域同源的病毒序列背景中具有完全功能。我们还描述了维斯纳病毒Rev的衍生物，其激活结构域有缺陷，在转染细胞中表现出反式显性负表型。这些维斯纳病毒Rev突变体可能在培育对这种具有农业重要性的逆转录病毒病原体有抗性的转基因动物方面有用。
