用吖啶黄或吖啶橙测定羧甲基纤维素钠的荧光猝灭法

Fluorescence quenching method for the determination of sodium carboxymethyl cellulose with acridine yellow or acridine orange.

作者信息

Liu Shao Pu, Sa Chen, Hu Xiao Li, Kong Ling

机构信息

School of Chemistry and Chemical Engineering, Southwest China Normal University, Chongqing 400715, China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2006 Jul;64(4):817-22. doi: 10.1016/j.saa.2005.08.009. Epub 2006 Feb 2.

DOI:10.1016/j.saa.2005.08.009

PMID:16458058

Abstract

In near neutral to weak basic media, sodium carboxymethyl cellulose (NaCMC) will dissociate to become a macro polymeric anion, which can react with acridine yellow (AY) or acridine orange (AO) to form an ion-association complex resulting in fluorescence quenching of the acridine dyes. The maximum fluorescence quenching wavelength is 505 nm (lambda(ex)=440 nm) for AY system and 530 nm (lambda(ex)=493 nm) for AO system, respectively. The fluorescence quenching values (DeltaF) are directly proportional to the concentrations of NaCMC and the linear ranges are 20.0-4000 microg/L for AY system and 20.0-7000 microg/L for AO system, separately. This method has high sensitivity and the detection limits for NaCMC are 58.0 microg/L (AY system) and 157.2 microg/L (AO system). The effects of coexistent substance have been investigated, and the results show that this method has a relatively good selectivity. A fluorescence quenching method for the determination of NaCMC based on the ion-association reactions of CMC polymeric anion with a basic acridine dye was developed. The method is sensitive, simple and fast.

摘要

在近中性至弱碱性介质中，羧甲基纤维素钠（NaCMC）会解离成为一种大分子聚合阴离子，它能与吖啶黄（AY）或吖啶橙（AO）反应形成离子缔合络合物，导致吖啶染料的荧光猝灭。对于AY体系，最大荧光猝灭波长分别为505 nm（激发波长λ(ex)=440 nm）；对于AO体系，最大荧光猝灭波长为530 nm（激发波长λ(ex)=493 nm）。荧光猝灭值（ΔF）与NaCMC的浓度成正比，AY体系的线性范围为20.0 - 4000 μg/L，AO体系的线性范围为20.0 - 7000 μg/L。该方法具有高灵敏度，NaCMC的检测限分别为58.0 μg/L（AY体系）和157.2 μg/L（AO体系）。研究了共存物质的影响，结果表明该方法具有较好的选择性。建立了一种基于CMC聚合阴离子与碱性吖啶染料的离子缔合反应测定NaCMC的荧光猝灭方法。该方法灵敏、简便、快速。