Schneider Manuela, Vogt Weisenhorn Daniela M, Seiler Alexander, Bornkamm Georg W, Brielmeier Markus, Conrad Marcus
Department of Comparative Medicine, GSF Research Centre for Environment and Health, Ingolstaedter Landstr. 1, D-85764 Neuherberg, Germany.
Gene Expr Patterns. 2006 Jun;6(5):489-94. doi: 10.1016/j.modgep.2005.11.002. Epub 2006 Feb 2.
The selenoenzyme phospholipid hydroperoxide glutathione peroxidase (PHGPx) is indispensable for murine embryonic development; yet, the cellular mechanisms leading to embryonic death around gastrulation are still unclear. To investigate PHGPx expression patterns during embryogenesis, we performed a detailed analysis that revealed a complex expression profile. Up to embryonic day 9.5, PHGPx was ubiquitously expressed, which was, albeit to a lower extent, maintained throughout later stages of embryogenesis. Notably, strong expression was frequently observed in epithelial tissue. A transient increase in PHGPx expression was detected in developing tissues, suggesting a crucial role for PHGPx in proliferation and differentiation. By semi-quantitative RT-PCR analysis we observed that the cytosolic form of PHGPx was present in embryonic and somatic tissues whereas the mitochondrial and nuclear forms were detectable only in testicular tissue. This strongly suggests that it is the cytosolic form of PHGPx that is indispensable for embryonic development.
硒酶磷脂氢过氧化物谷胱甘肽过氧化物酶（PHGPx）对小鼠胚胎发育不可或缺；然而，导致原肠胚形成期左右胚胎死亡的细胞机制仍不清楚。为了研究胚胎发生过程中PHGPx的表达模式，我们进行了详细分析，揭示了一个复杂的表达谱。直到胚胎第9.5天，PHGPx广泛表达，尽管程度较低，但在胚胎发生的后期阶段一直保持。值得注意的是，在上皮组织中经常观察到强表达。在发育中的组织中检测到PHGPx表达的短暂增加，表明PHGPx在增殖和分化中起关键作用。通过半定量RT-PCR分析，我们观察到PHGPx的胞质形式存在于胚胎组织和体细胞组织中，而线粒体和核形式仅在睾丸组织中可检测到。这强烈表明，对胚胎发育不可或缺的是PHGPx的胞质形式。
