Kissenpfennig Adrien, Malissen Bernard
Centre d'Immunologie de Marseille-Luminy, INSERM-CNRS-Université de la Méditerrannée, Parc Scientifique et Technologique de Luminy, Case 906, 13288 Marseille Cedex 09, France.
Trends Immunol. 2006 Mar;27(3):132-9. doi: 10.1016/j.it.2006.01.003. Epub 2006 Feb 3.
Langerhans cells (LCs) are prominent dendritic cells (DCs) in epithelia, but their role in immunity and tolerance is poorly defined. 'Knockin' mice expressing enhanced green fluorescent protein (EGFP) under the control of the langerin (CD207) gene were recently developed in order to discriminate epidermal LCs from other DC subsets and at the same time to track their dynamics under steady-state or inflammatory conditions in vivo. Additional knockin mice expressing a diphtheria toxin receptor fused to EGFP were used to conditionally ablate LCs and assess their role in triggering hapten-specific T cell effectors through skin immunization. We review the insights that have been provided by these various knockin mice and discuss gaps in our knowledge of LCs that need to be filled.
朗格汉斯细胞（LCs）是上皮组织中突出的树突状细胞（DCs），但其在免疫和耐受中的作用尚不清楚。最近开发了在朗格蛋白（CD207）基因控制下表达增强型绿色荧光蛋白（EGFP）的“敲入”小鼠，以便将表皮LCs与其他DC亚群区分开来，同时在体内稳态或炎症条件下追踪它们的动态变化。还使用了表达与EGFP融合的白喉毒素受体的额外敲入小鼠，有条件地消除LCs，并通过皮肤免疫评估它们在触发半抗原特异性T细胞效应器中的作用。我们回顾了这些不同敲入小鼠所提供的见解，并讨论了我们在LCs知识方面需要填补的空白。
