Elkord Eyad, Rowbottom Anthony W, Kynaston Howard, Williams Paul E
Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Cardiff, UK.
Clin Immunol. 2006 Jul;120(1):91-8. doi: 10.1016/j.clim.2005.12.008. Epub 2006 Feb 3.
Modest work has been performed to improve the sensitivity of residual disease detection or investigate the contribution that the immune system makes in controlling metastatic tumor growth, in particular, the frequency and biological actions of peptide-specific CD8+ T lymphocytes in limiting metastatic disease and/or maintaining remission. Fifty-three peripheral blood samples from 32 prostate cancer (PC) patients were investigated for the presence of circulating prostate-specific antigen (PSA)-expressing cells (CPECs) using a highly sensitive and specific assay combining immunomagnetic epithelial cell enrichment with nested RT-PCR of PSA mRNA. Using HLA-A2 tetramer complexes, frequency of CD8+ T cells specific for PSA-derived peptides was determined. Additionally, serum concentrations of PSA and testosterone were measured. CPECs were detected in 26% of peripheral blood samples from PC patients. CD8+ T cells specific for PSA-derived peptides were detected at low frequency in HLA-A2-positive PC patients. The correlation between these PSA-specific CD8+ T cells and residual prostate tumor cells and clinical measures was investigated. Our data suggest that frequency of PSA-specific CD8+ T cells is correlated to CPECs, but not to serum PSA level.
在提高残留疾病检测的敏感性或研究免疫系统在控制转移性肿瘤生长中所起的作用方面，尤其是在限制转移性疾病和/或维持缓解方面，肽特异性CD8 + T淋巴细胞的频率和生物学作用，所做的工作较少。使用一种高度敏感和特异的检测方法，将免疫磁珠上皮细胞富集与PSA mRNA的巢式RT-PCR相结合，对32例前列腺癌（PC）患者的53份外周血样本进行了循环前列腺特异性抗原（PSA）表达细胞（CPEC）的检测。使用HLA-A2四聚体复合物，测定了PSA衍生肽特异性CD8 + T细胞的频率。此外，还测量了血清PSA和睾酮的浓度。在PC患者26%的外周血样本中检测到了CPEC。在HLA-A2阳性的PC患者中，以低频检测到了PSA衍生肽特异性CD8 + T细胞。研究了这些PSA特异性CD8 + T细胞与残留前列腺肿瘤细胞及临床指标之间的相关性。我们的数据表明，PSA特异性CD8 + T细胞的频率与CPEC相关，但与血清PSA水平无关。
