LaRue Amanda C, Masuya Masahiro, Ebihara Yasuhiro, Fleming Paul A, Visconti Richard P, Minamiguchi Hitoshi, Ogawa Makio, Drake Christopher J
Department of Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, USA.
Exp Hematol. 2006 Feb;34(2):208-18. doi: 10.1016/j.exphem.2005.10.009.
Recent studies have reported that bone marrow cells can give rise to tissue fibroblasts. However, the bone marrow cell(s) that gives rise to fibroblasts has not yet been identified. In the present study, we tested the hypothesis that tissue fibroblasts are derived from hematopoietic stem cells (HSCs) in vivo.
These studies were conducted using mice whose hematopoiesis had been reconstituted by transplantation of a clonal population of cells derived from a single enhanced green fluorescent protein (EGFP)-positive HSC in conjunction with murine tumor models.
When tumors propagated in the transplanted mice were evaluated for the presence of EGFP(+) HSC-derived cells, two prominent populations of EGFP(+) cells were found. The first were determined to be fibroblasts within the tumor stromal capsule, a subset of which expressed type I collagen mRNA and alpha-smooth muscle actin. The second population was a perivascular cell associated with the CD31(+) tumor blood vessels.
These in vivo findings establish an HSC origin of fibroblasts.
近期研究报道骨髓细胞可分化为组织成纤维细胞。然而，产生成纤维细胞的骨髓细胞尚未被鉴定出来。在本研究中，我们检验了组织成纤维细胞在体内源自造血干细胞（HSC）这一假说。
这些研究使用的小鼠，其造血功能通过移植源自单个增强型绿色荧光蛋白（EGFP）阳性HSC的克隆细胞群体并结合小鼠肿瘤模型来重建。
当评估移植小鼠体内肿瘤中EGFP（+）HSC来源细胞的存在情况时，发现了两个主要的EGFP（+）细胞群体。第一个群体被确定为肿瘤基质囊内的成纤维细胞，其中一部分表达I型胶原mRNA和α平滑肌肌动蛋白。第二个群体是与CD31（+）肿瘤血管相关的血管周围细胞。
这些体内研究结果证实了成纤维细胞起源于HSC。
