Ganong B R, Delmore J P
Department of Biochemistry, University of Alabama, Birmingham 35294.
Anal Biochem. 1991 Feb 15;193(1):35-7. doi: 10.1016/0003-2697(91)90039-v.
Triton X-114 solutions separate above 22 degrees C into two immiscible aqueous phases. The more dense phase is enriched in detergent, and the less dense phase is depleted of detergent, relative to the original single phase. This phenomenon has been used to partition proteins according to hydrophobicity. The phase separation temperature is sensitive to the length of the polyoxyethylene headgroup. When Triton X-45, with a shorter headgroup, is mixed with Triton X-114 in various proportions, the phase transition temperature can be adjusted anywhere between 0 and 22 degrees C. Partitioning properties of the resulting mixtures are similar to those of Triton X-114 alone.
Triton X - 114溶液在22摄氏度以上会分离成两个互不相溶的水相。相对于原始单相，密度较大的相富含去污剂，而密度较小的相去污剂含量较低。这种现象已被用于根据疏水性对蛋白质进行分配。相分离温度对聚氧乙烯头基的长度敏感。当具有较短头基的Triton X - 45与Triton X - 114以不同比例混合时，相变温度可在0至22摄氏度之间的任何位置进行调节。所得混合物的分配特性与单独的Triton X - 114相似。
