Miyasaki K T, Song J P, Murthy R K
Section of Oral Biology, UCLA School of Dentistry, Center for the Health Sciences 90024.
Anal Biochem. 1991 Feb 15;193(1):38-44. doi: 10.1016/0003-2697(91)90040-z.
The human neutrophil lysosomal enzyme, myeloperoxidase (MPO), exists in three major and chromatographically distinct forms, MPO I, MPO II, and MPO III. We used cation-exchange medium-pressure liquid chromatography and kinetic microenzyme assay (or spectrophotometric monitoring) to analyze the secretion of MPO isoforms by neutrophils exposed to N-formylmethionylleucylphenylalanine (FMLP), digitonin, the ionophore A23187, and serum-opsonized zymosan A (SOZ). All three MPO isomers were released into the fluid phase after neutrophils were exposed to these secretagogues. A significant proportional increase in MPO I was released when neutrophils were stimulated with SOZ. MPO I was released in higher proportions than found in the whole cell constituency when neutrophils were stimulated with FMLP + cytochalasin B, A23187, and digitonin, but this was not statistically significant.
人类中性粒细胞溶酶体酶髓过氧化物酶（MPO）存在三种主要的、色谱性质不同的形式，即MPO I、MPO II和MPO III。我们使用阳离子交换中压液相色谱法和动力学微酶测定法（或分光光度监测）来分析暴露于N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）、洋地黄皂苷、离子载体A23187和血清调理酵母聚糖A（SOZ）的中性粒细胞分泌MPO同工型的情况。在中性粒细胞暴露于这些促分泌剂后，所有三种MPO异构体都释放到液相中。当用SOZ刺激中性粒细胞时，MPO I释放量有显著的比例增加。当用FMLP + 细胞松弛素B、A23187和洋地黄皂苷刺激中性粒细胞时，MPO I的释放比例高于全细胞组成中的比例，但这在统计学上不显著。
