Prakash Anil, Bhattacharyya D R, Mohapatra P K, Mahanta J
Regional Medical Research Centre, NE Region, Indian Council of Medical Research, Post Box no. 105, Dibrugarh-786 001, India.
J Environ Biol. 2005 Oct;26(4):719-23.
Anopheles philippinensis-nivipes complex mosquitoes, captured in outdoor human landing catches and light traps in human dwellings from four different sites in Assam state and adjoining areas, were examined (n=1670) for the presence of circumsporozoite antigen (CSA) through enzyme linked immunosorbent assays (ELISA), using species specific capture monoclonal antibodies, of Plasmodium falciparum and two Plasmodium vivax polymorphs (Pv 210 and VK 247). In ELISA, 28 pools were found positive for CSA that accounted for minimum sporozoite rate of 1.7% (95% CI 1.11-2.41). Twenty five percent (7/28) of the positive pools were reactive for P. falciparum and between the two polymorphs of P. vivax, VK 247 was predominant with 77% (20/26) of all P. vivax positive pools. Results were suggestive of most likely involvement of Anopheles philippinensis-nivipes complex mosquitoes in malaria transmission in north-east India.
从阿萨姆邦四个不同地点及毗邻地区的人类住所中，通过户外人饵诱捕法和光诱捕法捕获了菲律宾按蚊-尼氏按蚊复合组蚊子（n = 1670），利用恶性疟原虫和两种间日疟原虫多态体（Pv 210和VK 247）的种特异性捕获单克隆抗体，通过酶联免疫吸附测定（ELISA）检测环子孢子抗原（CSA）的存在情况。在ELISA检测中，发现28个样本池的CSA呈阳性，最低子孢子率为1.7%（95%置信区间1.11 - 2.41）。25%（7/28）的阳性样本池对恶性疟原虫有反应，在间日疟原虫的两种多态体中，VK 247占主导，占所有间日疟原虫阳性样本池的77%（20/26）。结果表明，菲律宾按蚊-尼氏按蚊复合组蚊子很可能参与了印度东北部的疟疾传播。
