van Vliet Jane, Crofts Linda A, Quinlan Kate G R, Czolij Robert, Perkins Andrew C, Crossley Merlin
School of Molecular and Microbial Biosciences, G08, University of Sydney, Sydney, NSW 2006, Australia.
Genomics. 2006 Apr;87(4):474-82. doi: 10.1016/j.ygeno.2005.12.011. Epub 2006 Feb 7.
The Sp/KLF transcription factors perform a variety of biological functions, but are related in that they bind GC-box and CACCC-box sequences in DNA via a highly conserved DNA-binding domain. A database homology search, using the zinc finger DNA-binding domain characteristic of the family, has identified human KLF17 as a new family member that is most closely related to KLFs 1-8 and 12. KLF17 appears to be the human orthologue of the previously reported mouse gene, zinc finger protein 393 (Zfp393), although it has diverged significantly. The DNA-binding domain is the most conserved region, suggesting that both the murine and the human forms recognize the same binding sites in DNA and may retain similar functions. We show that human KLF17 can bind G/C-rich sites via its zinc fingers and is able to activate transcription from CACCC-box elements. This is the first report of the DNA-binding characteristics and transactivation activity of human KLF17, which, together with the homology it displays to other KLF proteins, put it in the Sp/KLF family.
Sp/KLF转录因子具有多种生物学功能，但它们的相关之处在于，它们通过高度保守的DNA结合结构域与DNA中的GC盒和CACCC盒序列结合。利用该家族特有的锌指DNA结合结构域进行数据库同源性搜索，已确定人类KLF17是与KLFs 1-8和12关系最密切的新家族成员。KLF17似乎是先前报道的小鼠基因锌指蛋白393（Zfp393）的人类同源物，尽管它已经发生了显著分化。DNA结合结构域是最保守的区域，这表明小鼠和人类形式都能识别DNA中的相同结合位点，并可能保留相似的功能。我们发现人类KLF17可以通过其锌指结合富含G/C的位点，并能够激活来自CACCC盒元件的转录。这是关于人类KLF17的DNA结合特性和反式激活活性的首次报道，它与其他KLF蛋白的同源性使其属于Sp/KLF家族。
