Choi Sang-Haeng, Kim Il-Chul, Kim Dae-Soo, Kim Dae-Won, Chae Sung-Hwa, Choi Han-Ho, Choi Inho, Yeo Jung-Sou, Song Mi-Na, Park Hong-Seog
Genome Research Center, Korea Research Institute of Bioscience and Biotechnology, 52, Oun-dong, Yusong-gu, Daejeon 305-333, Korea.
Genomics. 2006 May;87(5):598-607. doi: 10.1016/j.ygeno.2005.12.012. Epub 2006 Feb 7.
We sequenced a 208-kb BAC clone spanning the bovine prion protein (PRNP) locus, and compared the genomic structure with that of human. As a result, we determined the precise breakpoint between the two syntenic genomes, located on the 5' UTR of the PRNP gene, and discovered two highly repetitive sequences near the breakpoint. Further analysis demonstrated that the genomic structure of three genes, PRNP, PRND, and RASSF2, within the syntenic region of the bovine genome is highly conserved in order and orientation. The PRNT locus was not found in bovine but is conserved in several primates, including human. Moreover, we confirmed that the bovine RASSF2 is composed of 10 exons, as is the human gene, showing some difference from a previous report. Our findings may provide useful clues for understanding the evolutional process in the PRNP locus and also the mechanism that allows TSE from cattle to infect humans.
我们对一个跨越牛朊病毒蛋白（PRNP）基因座的208-kb细菌人工染色体（BAC）克隆进行了测序，并将其基因组结构与人类的进行了比较。结果，我们确定了两个同线基因组之间的精确断点，该断点位于PRNP基因的5'非翻译区（UTR），并在断点附近发现了两个高度重复的序列。进一步分析表明，牛基因组同线区域内的三个基因PRNP、PRND和RASSF2的基因组结构在顺序和方向上高度保守。在牛中未发现PRNT基因座，但在包括人类在内的几种灵长类动物中是保守的。此外，我们证实牛RASSF2与人类基因一样由10个外显子组成，这与之前的报道有所不同。我们的发现可能为理解PRNP基因座的进化过程以及牛海绵状脑病（TSE）感染人类的机制提供有用的线索。
