Pichon-Santander Clotilde, Santander Patricio J, Scott A Ian
Department of Chemistry, Texas A&M University, PO Box 30012, College Station, TX 77842-3012, USA.
Bioorg Med Chem. 2006 Jun 1;14(11):3904-22. doi: 10.1016/j.bmc.2006.01.041. Epub 2006 Feb 7.
The specificity toward substrate analogs of the first two methyltransferases in the vitamin B(12) biosynthetic pathway was probed with 15 synthetic porphyrinogens. Several novel methylated chlorins and isobacteriochlorins were isolated and characterized, suggesting the same methylation sequence C-2>C-7>C-20 as for the natural substrate, uro'gen III. The results allow us to narrow down possible structural requirements concerning substrate recognition by the methyltransferase enzymes.
利用15种合成卟吩原对维生素B12生物合成途径中前两种甲基转移酶对底物类似物的特异性进行了探究。分离并表征了几种新型甲基化二氢卟酚和异菌二氢卟酚，这表明其甲基化顺序与天然底物尿卟吩原III相同，即C-2>C-7>C-20。这些结果使我们能够缩小甲基转移酶对底物识别的可能结构要求范围。
