Vermillion D L, Ernst P B, Collins S M
Intestinal Disease Research Unit, McMaster University, Hamilton, Ontario, Canada.
Gastroenterology. 1991 Jul;101(1):31-8. doi: 10.1016/0016-5085(91)90456-u.
Jejunal longitudinal muscle from Trichinella-infected Sprague-Dawley rats generates increased tension in vitro 6 days after infection. To investigate the extent to which this increase is dependent on T lymphocytes, smooth muscle contraction was examined in athymic rats (rnu/rnu) and in their euthymic (rnu/+) littermates 6 days after infection. In both thymus-bearing and athymic rats, the highest concentrations of Trichinella were found in the jejunum where mucosal myeloperoxidase activity was increased. Muscle from infected euthymic rats generated more tension to carbachol or 5-hydroxytryptamine than that of noninfected controls. In contrast, there was no difference between the responses of muscle from infected and control athymic animals. However, when athymic rats were reconstituted with splenic mononuclear cells, increased tension to carbachol or 5-hydroxytryptamine was observed after infection. T-lymphocyte activity was demonstrated by in vitro assays in euthymic or reconstituted rats but not in athymic rats. It is concluded that some changes in intestinal smooth muscle function following Trichinella spiralis infection in the rat are T lymphocyte dependent. These results support the concept of immunomodulation of intestinal smooth muscle function.
感染旋毛虫的斯普拉格-道利大鼠空肠纵肌在感染后6天体外产生的张力增加。为了研究这种增加在多大程度上依赖于T淋巴细胞，在感染后6天，对无胸腺大鼠（rnu/rnu）及其有胸腺的（rnu/+）同窝仔鼠的平滑肌收缩进行了检测。在有胸腺和无胸腺的大鼠中，旋毛虫浓度最高的部位均为空肠，此处黏膜髓过氧化物酶活性增加。感染的有胸腺大鼠的肌肉对卡巴胆碱或5-羟色胺产生的张力比未感染的对照组更大。相比之下，感染的和对照的无胸腺动物的肌肉反应没有差异。然而，当用脾单核细胞重建无胸腺大鼠时，感染后观察到对卡巴胆碱或5-羟色胺的张力增加。在有胸腺或重建的大鼠中通过体外试验证实了T淋巴细胞活性，但在无胸腺大鼠中未证实。得出的结论是，大鼠感染旋毛虫后肠道平滑肌功能的一些变化依赖于T淋巴细胞。这些结果支持肠道平滑肌功能免疫调节的概念。
