Malten Marco, Biedendieck Rebekka, Gamer Martin, Drews Ann-Christin, Stammen Simon, Buchholz Klaus, Dijkhuizen Lubbert, Jahn Dieter
Institute of Microbiology, Technical University Braunschweig, Spielmannstrasse 7, D-38106 Braunschweig, Germany.
Appl Environ Microbiol. 2006 Feb;72(2):1677-9. doi: 10.1128/AEM.72.2.1677-1679.2006.
A multiple vector system for the production and export of recombinant affinity-tagged proteins in Bacillus megaterium was developed. Up to 1 mg/liter of a His6-tagged or Strep-tagged Lactobacillus reuteri levansucrase was directed into the growth medium, using the B. megaterium esterase LipA signal peptide, and recovered by one-step affinity chromatography.
开发了一种用于在巨大芽孢杆菌中生产和输出重组亲和标签蛋白的多载体系统。利用巨大芽孢杆菌酯酶LipA信号肽,将高达1毫克/升的His6标签或链霉亲和素标签的罗伊氏乳杆菌蔗糖转化酶导入生长培养基,并通过一步亲和色谱法进行回收。