在蚊虫细胞系C6/36中分离出猫立克次体。
Isolation of Rickettsia felis in the mosquito cell line C6/36.
作者信息
Horta Maurício C, Labruna Marcelo B, Durigon Edison L, Schumaker Teresinha T S
机构信息
Faculty of Veterinary Medicine, University of Sao Paulo, São Paulo, SP 05508-270, Brazil.
出版信息
Appl Environ Microbiol. 2006 Feb;72(2):1705-7. doi: 10.1128/AEM.72.2.1705-1707.2006.
Abstract
We report the isolation and establishment of Rickettsia felis in the C6/36 cell line. Rickettsial growth was intense, always with 90 to 100% of cells being infected after few weeks. The rickettsial isolate was confirmed by testing infected cells by PCR and sequencing fragments of three major Rickettsia genes (gltA, ompB, and the 17-kDa protein gene).
摘要
我们报告了在C6/36细胞系中分离和建立猫立克次体的过程。立克次体生长旺盛，几周后总是有90%至100%的细胞被感染。通过对感染细胞进行PCR检测以及对三个主要立克次体基因（gltA、ompB和17-kDa蛋白基因）的片段进行测序，确认了立克次体分离株。
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