一种使用单克隆抗体检测人血浆和尿液中鸟苷 3',5'-环磷酸（cGMP）的酶联免疫吸附测定（ELISA）。

An enzyme-linked immunosorbent assay (ELISA) for guanosine 3',5'-cyclic monophosphate (cGMP) in human plasma and urine using monoclonal antibody.

作者信息

Tsugawa M, Moriwaki K, Iida S, Fujii H, Gomi M, Tarui S, Yamane R, Fujimoto M

机构信息

Second Department of Internal Medicine, Osaka University Medical School, Japan.

出版信息

J Immunoassay. 1991;12(2):263-76. doi: 10.1080/01971529108055071.

DOI:10.1080/01971529108055071

PMID:1646221

Abstract

An ELISA for cGMP in human plasma and urine using a monoclonal antibody is described. The monoclonal antibody was raised against succinyl cGMP conjugated to human serum albumin. The conjugate was adsorbed to the ELISA plate, giving an immobilized antigen approach which simplifies subsequent assay procedures. As low as 1.56 fmol/well of both plasma and urinary cGMP is measurable. Recoveries of added cGMP in plasma and urine were from 97% to 105%. Intra-assay coefficients of variation were less than 7.0% for plasma and 7.1% for urine samples. Inter-assay coefficients of variation for plasma and urine samples were less than 9.9% and 9.5%, respectively. The values obtained by ELISA correlated well with those by radioimmunoassay (RIA) (plasma: r = 0.96, n = 50; urine: r = 0.98, n = 60).

摘要

本文描述了一种使用单克隆抗体检测人血浆和尿液中cGMP的酶联免疫吸附测定法（ELISA）。该单克隆抗体是针对与人血清白蛋白偶联的琥珀酰cGMP产生的。将偶联物吸附到ELISA板上，采用固定抗原法，简化了后续的检测程序。血浆和尿液中cGMP的检测下限低至1.56 fmol/孔。血浆和尿液中添加的cGMP回收率在97%至105%之间。血浆样本的批内变异系数小于7.0%，尿液样本的批内变异系数小于7.1%。血浆和尿液样本的批间变异系数分别小于9.9%和9.5%。ELISA法获得的值与放射免疫测定法（RIA）获得的值相关性良好（血浆：r = 0.96，n = 50；尿液：r = 0.98，n = 60）。