抗人血管紧张素原单克隆抗体、其特性及在血管紧张素原酶联免疫吸附测定中的应用

Monoclonal antibodies against human angiotensinogen, their characterization and use in an angiotensinogen enzyme linked immunosorbent assay.

作者信息

Rubin I, Lykkegaard S, Olsen A A, Selmer J, Ballegaard M

机构信息

Department of Biochemistry A, Panum Institute, University of Copenhagen, Denmark.

出版信息

J Immunoassay. 1988;9(3-4):257-74. doi: 10.1080/01971528808053216.

DOI:10.1080/01971528808053216

PMID:2466873

Abstract

Monoclonal antibodies were produced against human angiotensinogen. An enzyme linked immunosorbent assay (ELISA) was developed using a high affinity monoclonal antibody as catching antibody and a polyclonal rabbit anti human angiotensinogen antibody as detecting antibody in a "sandwich" ELISA. Linear range of the ELISA was 15-450 pmol/l of human angiotensinogen. Intra- and inter- assay variation coefficients were in the range of 2% to 8%. A correlation coefficient, r = 0.97, (n = 20), with values obtained by radioimmunoassay. This correlation coefficient, obtained by using both normal and pregnant sera, confirmed that the ELISA fulfill the requirements for clinical useful assay. Characterization of the antibodies were performed with respect to affinity constant and epitopes.

摘要

制备了抗人血管紧张素原的单克隆抗体。开发了一种酶联免疫吸附测定法（ELISA），在“夹心”ELISA中，使用高亲和力单克隆抗体作为捕获抗体，兔抗人血管紧张素原多克隆抗体作为检测抗体。该ELISA法检测人血管紧张素原的线性范围为15 - 450 pmol/L。批内和批间变异系数在2%至8%范围内。相关系数r = 0.97（n = 20），与放射免疫测定法获得的值相关。通过使用正常血清和孕妇血清获得的该相关系数证实，该ELISA法符合临床实用检测的要求。对抗体的亲和力常数和表位进行了表征。