Hritz Istvan, Kuester Doerthe, Vieth Michael, Herszenyi Laszlo, Stolte Manfred, Roessner Albert, Tulassay Zsolt, Wex Thomas, Malfertheiner Peter
Department of Gastroenterology, Hepatology and Infectious Diseases, Faculty of Medicine, Semmelweis University, Budapest, Hungary.
Eur J Gastroenterol Hepatol. 2006 Mar;18(3):277-82. doi: 10.1097/00042737-200603000-00008.
Secretory leukocyte protease inhibitor (SLPI) represents a multifunctional protein of the gastrointestinal mucosa exerting antimicrobial and anti-inflammatory effects. SLPI expression is generally induced during inflammation; however, Helicobacter pylori-mediated gastritis is associated with significantly decreased antral SLPI levels. The aim of the study was to investigate whether SLPI downregulation of gastric mucosa represents a specific phenomenon of H. pylori infection or is generally linked to gastric inflammation.
SLPI expression was retrospectively analysed by immunohistochemistry in 85 paraffin-embedded samples: H. pylori-induced (n=13), non-steroidal anti-inflammatory drug (NSAID)-enhanced (n=18), autoimmune (n=11), lymphocytic gastritis (n=26) and H. pylori-negative controls (n=17). The intensity of the staining was semiquantitatively analysed using an immunoreactivity score. Statistical analysis of differences was performed using an analysis of variance test.
In comparison with the control group, the SLPI expression of antral mucosa in H. pylori-mediated and lymphocytic gastritis was significantly lower (P<0.001), whereas epithelial SLPI expression was not affected in NSAID-enhanced and autoimmune gastritis either in the antrum or corpus, respectively. Both the H. pylori-mediated and lymphocytic gastritis revealed a significantly lower expression of SLPI in infiltrating immune cells (P<0.01), whereas immune cells infiltrating the corpus in autoimmune gastritis showed higher SLPI levels than the immune cells of other groups (P<0.03).
The local downregulation of SLPI in antral mucosa is specifically linked to H. pylori infection and is not a general phenomenon of gastric inflammation.
分泌型白细胞蛋白酶抑制剂（SLPI）是胃肠道黏膜的一种多功能蛋白，具有抗菌和抗炎作用。SLPI表达通常在炎症过程中被诱导；然而，幽门螺杆菌介导的胃炎与胃窦部SLPI水平显著降低有关。本研究的目的是调查胃黏膜中SLPI下调是幽门螺杆菌感染的特异性现象还是与胃炎普遍相关。
通过免疫组织化学对85个石蜡包埋样本进行回顾性分析，这些样本包括幽门螺杆菌感染所致（n = 13）、非甾体抗炎药（NSAID）诱发（n = 18）、自身免疫性（n = 11）、淋巴细胞性胃炎（n = 26）以及幽门螺杆菌阴性对照（n = 17）。使用免疫反应性评分对染色强度进行半定量分析。采用方差分析检验对差异进行统计学分析。
与对照组相比，幽门螺杆菌介导的胃炎和淋巴细胞性胃炎中胃窦黏膜的SLPI表达显著降低（P < 0.001），而在NSAID诱发的胃炎和自身免疫性胃炎中，胃窦和胃体上皮的SLPI表达均未受影响。幽门螺杆菌介导的胃炎和淋巴细胞性胃炎中浸润免疫细胞的SLPI表达均显著降低（P < 0.01），而自身免疫性胃炎胃体中浸润的免疫细胞SLPI水平高于其他组的免疫细胞（P < 0.03）。
胃窦黏膜中SLPI的局部下调与幽门螺杆菌感染特异性相关，并非胃炎的普遍现象。
