Mao Wei, Chen Jie, Peng Tie-Li, Yin Xiao-Fei, Chen Lian-Zhou, Chen Min-Hu
The First Affiliated Hospital of Sun Yat-Sen University, Department of Gastroenterology, Guangzhou, China.
Turk J Gastroenterol. 2012 Jun;23(3):212-9. doi: 10.4318/tjg.2012.0345.
BACKGROUND/AIMS: Gastrokine-1 is a novel protein that plays an important role in the maintenance of the integrity of the gastric mucosa. However, whether Helicobacter pylori infection and non-steroidal anti-inflammatory drugs, which are known to cause gastric mucosal injuries, affect gastrokine-1 expression in the gastric mucosa is unknown. The aim of the present study was to determine gastric mucosal expression of gastrokine-1 in patients with Helicobacter pylori infection or long-term non-steroidal anti-inflammatory drug administration.
A total of 40 patients with functional dyspepsia (20 with Helicobacter pylori-negative chronic gastritis, and 20 with Helicobacter pylori-positive chronic gastritis), and 37 Helicobacter pylori-negative long-term non-steroidal anti-inflammatory drug users (26 with aspirin, 11 with selective cyclooxygenase-2 inhibitors) were selected. In addition, 20 Helicobacter pylori-negative healthy volunteers were recruited as controls. All subjects underwent endoscopies with biopsies taken from the antrum and the sites with lesions. Gastric mucosal changes were detected endoscopically and histologically, and gastrokine-1 protein expression in the antral mucosa was analyzed by immunohistochemistry.
Expression of gastrokine-1 protein was decreased in Helicobacter pylori-positive chronic gastritis compared with Helicobacter pylori-negative subjects and the healthy controls. Similarly, gastrokine-1 expression in non-steroidal anti-inflammatory drug users was also decreased, compared with the healthy controls, but there was no significant difference in gastrokine-1 expression between the aspirin group and selective cyclooxygenase-2 inhibitor group. Moreover, gastrokine-1 expression levels tended to be associated with the severity of chronic gastritis.
Both Helicobacter pylori infection and long-term non-steroidal anti-inflammatory drug administration downregulate gastrokine-1 expression in the gastric mucosa, which may contribute to the gastric mucosal injuries induced by these two factors.
背景/目的：胃动素-1是一种新型蛋白质，在维持胃黏膜完整性方面发挥重要作用。然而，已知会导致胃黏膜损伤的幽门螺杆菌感染和非甾体抗炎药是否会影响胃黏膜中胃动素-1的表达尚不清楚。本研究的目的是确定幽门螺杆菌感染患者或长期服用非甾体抗炎药患者胃黏膜中胃动素-1的表达情况。
共选取40例功能性消化不良患者（20例幽门螺杆菌阴性慢性胃炎患者和20例幽门螺杆菌阳性慢性胃炎患者）以及37例幽门螺杆菌阴性长期服用非甾体抗炎药的患者（26例服用阿司匹林，11例服用选择性环氧化酶-2抑制剂）。此外，招募20例幽门螺杆菌阴性健康志愿者作为对照。所有受试者均接受内镜检查，并从胃窦和病变部位取活检组织。通过内镜和组织学检测胃黏膜变化，采用免疫组织化学方法分析胃窦黏膜中胃动素-1蛋白的表达。
与幽门螺杆菌阴性受试者和健康对照相比，幽门螺杆菌阳性慢性胃炎患者胃动素-1蛋白表达降低。同样，与健康对照相比，非甾体抗炎药使用者的胃动素-1表达也降低，但阿司匹林组和选择性环氧化酶-2抑制剂组之间胃动素-1表达无显著差异。此外，胃动素-1表达水平往往与慢性胃炎的严重程度相关。
幽门螺杆菌感染和长期服用非甾体抗炎药均下调胃黏膜中胃动素-1的表达，这可能导致这两种因素引起的胃黏膜损伤。
