Akpogomeh B A, Johns E J
Department of Physiology, The Medical School, Birmingham, UK.
J Hypertens. 1991 Apr;9(4):373-84. doi: 10.1097/00004872-199104000-00010.
Selective alpha-adrenoceptor and calcium antagonists have been used to determine both the alpha 1-adrenoceptor subtype and the extracellular calcium requirement for renal nerve-mediated antinatriuresis and antidiuresis in deoxycorticosterone acetate (DOCA)-salt and two-kidney, one clip (2K1C) Goldblatt hypertensive rats. Stimulation of the renal nerves at low frequencies reduced urine flow and absolute and fractional sodium excretions by 40-60% in pentobarbitone anaesthetized control, sham-operated, DOCA-salt and 2K1C Goldblatt hypertensive rats. Administration of prazosin, but not idazoxan, inhibited the renal nerve-induced excretory responses in both models of hypertension, a result compatible with the involvement of alpha 1-adrenoceptors. By contrast, the calcium antagonist inhibited the renal nerve-dependent antinatriuresis and antidiuresis in DOCA-salt but not 2K1C Goldblatt hypertensive rats. These results showed that the renal nerves mediated their action via alpha 1-adrenoceptors, but that the postreceptor responses were dependent on extracellular calcium in DOCA-salt but not 2K1C Goldblatt rats.
选用α-肾上腺素能受体拮抗剂和钙拮抗剂，以确定在醋酸脱氧皮质酮（DOCA）-盐处理的大鼠以及两肾一夹（2K1C）型Goldblatt高血压大鼠中，肾神经介导的钠排泄减少和利尿减少所涉及的α1-肾上腺素能受体亚型及细胞外钙需求。在戊巴比妥麻醉的对照、假手术、DOCA-盐处理及2K1C型Goldblatt高血压大鼠中，低频刺激肾神经可使尿流量、绝对钠排泄量和分数钠排泄量减少40%-60%。在两种高血压模型中，给予哌唑嗪而非咪唑克生可抑制肾神经诱导的排泄反应，这一结果与α1-肾上腺素能受体的参与相符。相比之下，钙拮抗剂可抑制DOCA-盐处理大鼠中肾神经依赖性的钠排泄减少和利尿减少，但对2K1C型Goldblatt高血压大鼠无效。这些结果表明，肾神经通过α1-肾上腺素能受体介导其作用，但在DOCA-盐处理大鼠中，受体后反应依赖于细胞外钙，而在2K1C型Goldblatt大鼠中则不然。
