Czéh B, Hajnal A, Seress L
Clinical Neurobiology Laboratory, German Primate Center, Göttingen, Germany.
Prague Med Rep. 2005;106(3):261-74.
The present study aimed to asses the total number and distribution of the NADPH-diaphorase-positive non-pyramidal neurons in Ammon's horn and dentate gyrus of rat hippocampal formation. Cell bodies were counted according to the "disector" principle. The total numbers varied from 27 000 to 32 400. In all strains, approximately one third of the NADPH-diaphorase-reactive non-principal cells were found in the dentate gyrus and the remaining two thirds were within the Ammon's horn. Analysis of the dorsoventral differences revealed that approximately 70% of NADPH-diaphorase-positive cells were in the dorsal and 30% in the ventral hippocampus. Distribution of NADPH-diaphorase-reactive cells in the different layers of the dentate gyrus and Ammon's horn was similar in all strains. Double-labelling studies revealed colocalization of NADPH-diaphorase with calretinin, but none with calbindin or parvalbumin. NADPH-diaphorase-positive neurons appear to form the largest chemically identified subpopulation of the GABAergic inhibitory cell population of the hippocampal formation.
本研究旨在评估大鼠海马结构的海马角和齿状回中烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）阳性的非锥体神经元的总数及分布。根据“间隔互证法”对细胞体进行计数。总数在27000至32400之间。在所有品系中，约三分之一的NADPH-d反应性非主细胞位于齿状回，其余三分之二则在海马角内。对背腹差异的分析显示，约70%的NADPH-d阳性细胞位于背侧海马，30%位于腹侧海马。在所有品系中，NADPH-d反应性细胞在齿状回和海马角不同层中的分布相似。双重标记研究显示NADPH-d与钙视网膜蛋白共定位，但与钙结合蛋白或小白蛋白无共定位。NADPH-d阳性神经元似乎构成了海马结构中GABA能抑制性细胞群中最大的化学鉴定亚群。
