Qi Dahu, Berger Andrew J
University of Rochester, Rochester, New York 14627, USA.
Appl Opt. 2006 Jan 20;45(3):489-94. doi: 10.1364/ao.45.000489.
The enhancement of a dissolved chemical's Raman scattering by a liquid-core optical fiber (LCOF) geometry is absorption dependent. This dependence leads to a disruption of the usual linear correlation between chemical concentration and Raman peak area. To recover the linearity, we augmented a standard LCOF Raman spectroscopy system with spectrophotometric capabilities, permitting sequential measurements of Raman and absorption spectra within the LCOF. Measurements of samples with identical Raman-scatterer concentrations but different absorption coefficients are described. Using the absorption values, we reduced variations in the measured Raman intensities from 60% to less than 1%. This correction method should be important for LCOF-based Raman spectroscopy of sample sets with variable absorption coefficients, such as urine and blood serum from multiple patients.
液芯光纤（LCOF）结构对溶解化学物质拉曼散射的增强作用取决于吸收情况。这种依赖性导致化学浓度与拉曼峰面积之间通常的线性相关性被破坏。为了恢复线性关系，我们在标准的LCOF拉曼光谱系统中增加了分光光度测量功能，从而能够在LCOF内依次测量拉曼光谱和吸收光谱。文中描述了对具有相同拉曼散射体浓度但不同吸收系数的样品的测量。利用吸收值，我们将测得的拉曼强度变化从60%降低到了1%以下。对于基于LCOF的、用于测量具有可变吸收系数的样品集（如多名患者的尿液和血清）的拉曼光谱而言，这种校正方法应该具有重要意义。
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