Qi Dahu, Berger Andrew J
University of Rochester, The Institute of Optics, Department of Physics and Astronomy, Rochester, New York 14627, USA.
J Biomed Opt. 2005 May-Jun;10(3):031115. doi: 10.1117/1.1917842.
We describe the use of a Teflon-AF liquid core optical fiber (LCOF) geometry to enhance the collection of Raman scattering from the biochemical creatinine, dissolved in water and in urine. At short integration times, where shot noise is most troublesome, the enhanced signal leads to greater accuracy in estimating the creatinine concentration from the spectrum. At longer integration times, instabilities in the LCOF geometry manifest themselves, and the predictions are the same as or worse than those from standard cuvette-based spectral measurements. Photobleaching of fluorescence from urine is more extensive and more stable in the LCOF as well. Starting from the measured enhancement of a major creatinine Raman band, we calculate the expected ratio of prediction errors obtained using the two geometries, and it agrees closely with the observed ratio. These results indicate that Raman spectroscopy with these Teflon-AF LCOFs is stable enough for quantitative concentration predictions, accurate to a few percent of the concentration range spanned.
我们描述了使用聚四氟乙烯 - AF 液芯光纤（LCOF）结构来增强对溶解于水和尿液中的生化物质肌酐的拉曼散射的收集。在短积分时间下，散粒噪声最为棘手，增强后的信号能在从光谱估计肌酐浓度时带来更高的准确性。在较长积分时间下，LCOF 结构中的不稳定性会显现出来，其预测结果与基于标准比色皿的光谱测量结果相同或更差。尿液中荧光的光漂白在 LCOF 中也更为广泛且更稳定。从测量到的主要肌酐拉曼峰的增强情况出发，我们计算了使用这两种结构获得的预测误差的预期比值，它与观察到的比值非常吻合。这些结果表明，使用这些聚四氟乙烯 - AF LCOF 进行拉曼光谱分析对于定量浓度预测足够稳定，在跨越的浓度范围内准确到百分之几。
