Bailey D, Marks A, Stratis M, Baumal R
Department of Pathology, Toronto General Hospital, Canada.
Mod Pathol. 1991 Mar;4(2):167-71.
Antibody to placental alkaline phosphatase (PLAP) has been previously used in immunoperoxidase (IMP) staining studies of germ cell tumors and intratubular malignant germ cells (ITMGC) of the testis, the latter believed to be the precursor of these tumors. In this study, we compared staining by IMP using monoclonal antibody (mAb) and polyclonal antibody to PLAP with that seen using a mAb, M2A, which was previously shown to react with testicular seminomas and ITMGC. Antibody to PLAP and M2A reacted with different cellular components, as assessed by IMP staining of placenta and prepubertal testis and by Western blotting of seminoma lysates. Antibody to PLAP stained pure seminomas (seven of seven), pure embryonal carcinomas (four of four), and the seminoma (three of three) and embryonal carcinoma (six of six) components of mixed testicular germ cell tumors. M2A stained pure seminomas (26 of 26) and the seminoma component (three of three) of the mixed tumors, but failed to stain pure embryonal carcinomas (zero of four) or the embryonal carcinoma component (zero of five) of the mixed tumors. Both antibody to PLAP and M2A stained ITMGC of the testis. Since M2A stained seminomas and ITMGC but not embryonal carcinomas, seminomas would appear to be more closely related to ITMGC than embryonal carcinomas. This result has led us to speculate on the histogenesis of testicular germ cell tumors.
胎盘碱性磷酸酶（PLAP）抗体先前已用于生殖细胞肿瘤和睾丸管内恶性生殖细胞（ITMGC）的免疫过氧化物酶（IMP）染色研究，后者被认为是这些肿瘤的前体。在本研究中，我们将使用单克隆抗体（mAb）和多克隆抗体针对PLAP的IMP染色与使用mAb M2A的染色进行了比较，先前已证明M2A与睾丸精原细胞瘤和ITMGC发生反应。通过胎盘和青春期前睾丸的IMP染色以及精原细胞瘤裂解物的蛋白质印迹分析评估，针对PLAP的抗体和M2A与不同的细胞成分发生反应。针对PLAP的抗体对纯精原细胞瘤（7/7）、纯胚胎癌（4/4）以及混合性睾丸生殖细胞肿瘤的精原细胞瘤成分（3/3）和胚胎癌成分（6/6）进行了染色。M2A对纯精原细胞瘤（26/26）和混合性肿瘤的精原细胞瘤成分（3/3）进行了染色，但未能对纯胚胎癌（0/4）或混合性肿瘤的胚胎癌成分（0/5）进行染色。针对PLAP的抗体和M2A均对睾丸的ITMGC进行了染色。由于M2A对精原细胞瘤和ITMGC进行了染色，但未对胚胎癌进行染色，因此精原细胞瘤似乎比胚胎癌与ITMGC的关系更为密切。这一结果促使我们对睾丸生殖细胞肿瘤的组织发生进行推测。
