Burke A P, Mostofi F K
Department of Genitourinary Pathology, Armed Forces Institute of Pathology, Washington DC 20306-6000.
Hum Pathol. 1988 Jun;19(6):663-70. doi: 10.1016/s0046-8177(88)80172-2.
Two hundred three testicular germ cell tumors were studied immunohistochemically for the presence of placental alkaline phosphatase (PLAP). Special emphasis was placed on the pattern and incidence of positive staining of intratubular malignant germ cells (ITMGCs) adjacent to tumors. 99% of cases with adjacent ITMGCs showed a positive staining reaction in some or all IT-MGCs present. Other germ cell elements showed at least a focal positive staining reaction in the following proportions: seminomas, 96%; embryonal carcinomas, 96%; yolk sac tumors, 25%; mature teratomas, 5%; immature teratomas, 4%; choriocarcinomas, 45%; and syncytiotrophoblasts, 43%. The staining pattern for seminomas tended to be diffuse, whereas for embryonal carcinomas the staining pattern was more focal. Yolk sac tumors stained inconsistently for PLAP and a positive reaction was limited to a small percentage of cells. Syncytiotrophoblasts, singly or in choriocarcinomas, also showed variable positivity. These results corroborate the fact that PLAP is a sensitive marker for ITMGC, seminoma, and embryonal carcinoma.
对203例睾丸生殖细胞肿瘤进行免疫组化研究，以检测胎盘碱性磷酸酶（PLAP）的存在情况。特别关注肿瘤旁的管内恶性生殖细胞（ITMGCs）的阳性染色模式和发生率。99%存在相邻ITMGCs的病例中，部分或全部IT-MGCs呈现阳性染色反应。其他生殖细胞成分呈现至少局灶性阳性染色反应的比例如下：精原细胞瘤，96%；胚胎癌，96%；卵黄囊瘤，25%；成熟畸胎瘤，5%；未成熟畸胎瘤，4%；绒毛膜癌，45%；合体滋养层细胞，43%。精原细胞瘤的染色模式倾向于弥漫性，而胚胎癌的染色模式更具局灶性。卵黄囊瘤对PLAP染色不一致，阳性反应仅限于一小部分细胞。合体滋养层细胞单独或在绒毛膜癌中也显示出不同程度的阳性。这些结果证实了PLAP是ITMGC、精原细胞瘤和胚胎癌的敏感标志物这一事实。
