Pang S C, Sarda I R, Wigle D A, Zhou Z J, Jennings D B, Flynn T G
Department of Anatomy, Queen's University, Kingston, Ontario, Canada.
Regul Pept. 1991 Mar 26;33(1):1-9. doi: 10.1016/0167-0115(91)90009-6.
Plasma clearance and tissue binding of atrial natriuretic peptide (ANP) and iso-ANP were compared in Inactin-anaesthetized rats. It was found that the plasma half-life of iso-ANP was comparable to ANP. Appearance of trichloroacetic acid-soluble radioactivity of iso-ANP in the plasma was considerably slower than that of ANP, suggesting that the metabolic process of these two peptides may be different. Although the binding distribution of these two peptides was similar, the total binding of iso-ANP to organs other than the kidney was much lower. The kidney, lung, heart and adrenal gland appeared to be major target organs for iso-ANP. Autoradiography showed that iso-ANP bound specifically to the renal glomerulus and proximal part of the proximal tubule. This latter binding site in the kidney was not apparent with ANP, suggesting that iso-ANP may exerts its physiological action at different sites in this organ.
在戊巴比妥钠麻醉的大鼠中比较了心房利钠肽（ANP）和异ANP的血浆清除率及组织结合情况。发现异ANP的血浆半衰期与ANP相当。异ANP的三氯乙酸可溶性放射性在血浆中的出现比ANP慢得多，这表明这两种肽的代谢过程可能不同。虽然这两种肽的结合分布相似，但异ANP与肾脏以外器官的总结合量要低得多。肾脏、肺、心脏和肾上腺似乎是异ANP的主要靶器官。放射自显影显示异ANP特异性结合于肾小球和近端小管的近端部分。ANP在肾脏中的后一个结合位点不明显，这表明异ANP可能在该器官的不同位点发挥其生理作用。
