一种用于布氏锥虫亚种的新型半限定培养基。
A new semi-defined medium for Trypanosoma brucei sspp.
作者信息
Brun R, Jenni L
出版信息
Acta Trop. 1977 Mar;34(1):21-33.
PMID:16465
Abstract
A new, easy to prepare semi-defined medium for the cultivation of trypanosomes of the T. brucei complex is introduced. Containing the two commercially available media MEM (Minimum Essential Medium) and Medium 199 TC45 as well as 10% inactivated foetal calf serum (fcs), the medium supports optimum growth and direct adaptation of bloodstream forms. Growth characteristics, glucose uptake, amino acid utilization and the ultrastructure of trypanosomes grown in this medium are described briefly.
摘要
本文介绍了一种新型、易于制备的半限定培养基，用于培养布氏锥虫复合体的锥虫。该培养基含有两种市售培养基MEM（最低基本培养基）和199 TC45培养基以及10%热灭活胎牛血清（FCS），支持血流形式锥虫的最佳生长和直接适应。简要描述了在此培养基中生长的锥虫的生长特性、葡萄糖摄取、氨基酸利用和超微结构。
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