Cunningham I, Kaminsky R
J Parasitol. 1986 Dec;72(6):944-8.
When procyclic trypanosomes of Trypanosoma brucei brucei and Trypanosoma brucei rhodesiense were cultivated in Nunclon 25 cm2 flasks at 27 C in a liquid medium containing various tissue explants of Phormia regina Meigen, some of them developed into forms infective for mice. The infective stages were present at various periods of up to 29 days when the cultures were terminated. Larger numbers of explants of head-salivary glands than the other tissues used were required to produce infections. Infectivity titrations on trypanosome suspensions of T. b. brucei TRUM 252 and T. b. rhodesiense TRUM 497 indicated that only a small proportion of the populations was infective. Mice were rarely infected with trypanosomes grown in medium without explants. Only 1 mouse of the 11 inoculated developed a parasitemia from a control culture of T. b. rhodesiense TRUM 545. A few trypanosomes resembling epimastigotes and metacyclic forms were seen in stained samples of infective inocula.
当布氏布氏锥虫和布氏罗德西亚锥虫的前循环型锥虫在Nunclon 25 cm²培养瓶中于27℃下，在含有丽蝇（Phormia regina Meigen）各种组织外植体的液体培养基中培养时，其中一些会发育成可感染小鼠的形态。当培养终止时，感染阶段在长达29天的不同时期出现。与使用的其他组织相比，需要更多数量的头部唾液腺外植体才能产生感染。对布氏布氏锥虫TRUM 252和布氏罗德西亚锥虫TRUM 497的锥虫悬液进行感染性滴定表明，只有一小部分群体具有感染性。在没有外植体的培养基中生长的锥虫很少感染小鼠。在接种的11只小鼠中，只有1只从布氏罗德西亚锥虫TRUM 545的对照培养物中出现了寄生虫血症。在感染性接种物的染色样本中可见一些类似上鞭毛体和循环后期形态的锥虫。
