Wang Li-Chun, Zhao Lian-San, Tang Hong
Center of Infectious Diseases, Key Laboratory of Biotherapy for Human Diseases of Ministry of Education, West China Hospital, Sichuan University, Chengdu.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2006 Jan;26(1):63-7.
To observe the effect of Ganzhifu (GZF), a Chinese compound formula for Warming-yang on experimental hepatic fibrosis, and to explore its mechanism.
Fifty-nine Wistar rats were divided into the normal control group and the model group. After the hepatic fibrosis model was established in rats by subcutaneous injection of CCl4 and feeding 10% alcohol in the model group, the rats were randomly sub-divided into the Ganzhifu group, colchicine group and model group, they were treated with Ganzhifu, colchicine and normal saline of same volume once a day respectively for 30 days as one treatment course. The serum liver function and hepatic fibrosis indexes, liver tissue contents of hydroxyproline (Hyp), malondialdehyde (MDA) and superoxide dismutase (SOD) activity were measured at the end of the experiment. Histologic changes of liver tissue were observed and scored by the modified inflammatory activity and fibrosis semiquantative scoring system (SSS), and collagen type I , III and IV in liver tissue were stained and semiquantitive analysis conducted.
In the Ganzhifu group, liver function and inflammatory activity and fibrosis SSS scores were markedly improved, indexes of hepatic fibrosis, liver contents of Hyp, MDA and collagen type I, III and IV were significantly decreased, and the SOD activity was increased, the changes were more significant as compared with those in the model group. The protective effect of Ganzhifu against hepatic fibrosis was the same as that of colchicine.
Ganzhifu can alleviate or reverse hepatic fibrosis in experimental hepatic fibrosis, and antioxidation may be its mechanism.
观察温阳复方肝脂复（GZF）对实验性肝纤维化的影响并探讨其作用机制。
59只Wistar大鼠分为正常对照组和模型组。模型组大鼠经皮下注射四氯化碳并喂养10%乙醇建立肝纤维化模型后，再随机分为肝脂复组、秋水仙碱组和模型组，分别给予肝脂复、秋水仙碱及等体积生理盐水灌胃，每日1次，共30天为1个疗程。实验结束时测定血清肝功能及肝纤维化指标、肝组织羟脯氨酸（Hyp）、丙二醛（MDA）含量及超氧化物歧化酶（SOD）活性。采用改良的炎症活动度和纤维化半定量评分系统（SSS）观察并评分肝组织学变化，对肝组织Ⅰ、Ⅲ、Ⅳ型胶原进行染色并进行半定量分析。
肝脂复组大鼠肝功能、炎症活动度及纤维化SSS评分明显改善，肝纤维化指标、肝组织Hyp、MDA含量及Ⅰ、Ⅲ、Ⅳ型胶原明显降低，SOD活性升高，与模型组比较差异更显著。肝脂复抗肝纤维化作用与秋水仙碱相当。
肝脂复可减轻或逆转实验性肝纤维化，抗氧化作用可能是其作用机制。
