Liu Ying, Liu Ping, Hu Yi-Yang, Xu Lieming, Wang Lei, Mu Yongping, Du Guangli
Technology, Shanghai University of Traditional Chinese Medicine, Shanghai (201203).
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2006 Mar;26(3):224-7.
To investigate the effect of Fuzheng Huayu decoction (FHD) intervention on hepatic fibrosis.
Wistar rats were randomly divided into 3 groups: rats in the normal group only treated with subcutaneous injection of saline, rats in the model group and the FHD group were made into hepatic fibrosis by subcutaneous injection of 40% carbon tetrachloride (CCl4)-olive solution and then those in the FHD group were treated with FHD by gastric perfusion after modeling. Liver samples of the rats were obtained for routine pathological observation, hydroxyproline determination and proteome quantitative determination. After then, the proteome profile was obtained through 2-dimensional electrophoresis and silver staining, and analyzed. More than 30 proteins with different expression were identified by MALDI-TOF-MS.
(1) The integral response of vital movement such as body weight and activity of hepatic fibrosis declined in the CCl4 induced liver fibrosis rats; (2) Liver fibrosis were associated with abnormal metabolism; (3) There were four material metabolism-related protein showed by hepatic proteome mass spectrography, which expressed different between the normal and the fibrotic rats, i. e. the perchloric acid soluble protein, the phosphatidylinositol transferase, the phosphoglycerate kinase and the endoplasmic reticulum-60 protease; (4) The expressions of the above-mentioned four proteins in the FHD group were nearly the same as those of normal level.
(1) Liver fibrosis is accompanied with abnormal protein synthesis and decomposition, as well as the enhanced activity of glycolysis; (2) The existence of metabolism-related proteins is one of the elements for the liver in regulating metabolism; (3) The regulation on the expressions of metablism-related proteins is one of the pathways for FHD to exert its anti-hepatic fibrosis effect.
探讨扶正化瘀方（FHD）干预对肝纤维化的影响。
将Wistar大鼠随机分为3组：正常组大鼠仅皮下注射生理盐水，模型组和FHD组大鼠皮下注射40%四氯化碳（CCl4）-橄榄油溶液制备肝纤维化模型，造模后FHD组大鼠经胃灌注FHD进行治疗。取大鼠肝脏样本进行常规病理观察、羟脯氨酸测定和蛋白质组定量测定。然后通过二维电泳和银染获得蛋白质组图谱并进行分析。采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）鉴定30余种差异表达蛋白。
（1）CCl4诱导的肝纤维化大鼠体重、活动等生命活动整体反应下降；（2）肝纤维化与代谢异常有关；（3）肝脏蛋白质组质谱显示有4种物质代谢相关蛋白，在正常大鼠和纤维化大鼠中表达不同，即高氯酸可溶性蛋白、磷脂酰肌醇转移酶、磷酸甘油酸激酶和内质网-60蛋白酶；（4）上述4种蛋白在FHD组中的表达水平与正常水平相近。
（1）肝纤维化伴有蛋白质合成与分解异常以及糖酵解活性增强；（2）代谢相关蛋白的存在是肝脏调节代谢的要素之一；（3）调节代谢相关蛋白的表达是FHD发挥抗肝纤维化作用的途径之一。
