正常和成纤维细胞转化细胞膜中脂肪酸链的柔韧性
Fatty acid chain flexibility in the membranes of normal and transformed fibroblasts.
作者信息
Gaffney B J
出版信息
Proc Natl Acad Sci U S A. 1975 Feb;72(2):664-8. doi: 10.1073/pnas.72.2.664.
Abstract
Fatty acid spin labels were used to measure the inherent flexibility of the lipid acyl chains in intact cell membranes of 3T3 mouse fibroblasts and several varieties of transformed 3T3 cells. No significant differences in inherent lipid flexibility were detected in the normal and transformed mouse fibroblasts. These results are compared with similar results obtained earlier for chick embryo fibroblasts. For the cells studied, a difference in the mobility of membrane glycoproteins of normal and transformed fibroblasts has either been demonstrated directly or inferred from differential agglutinability by other investigations. Therefore, a basis for correlating glycoprotein mobility and lipid motion in these cells was not found.
摘要
脂肪酸自旋标记物被用于测量3T3小鼠成纤维细胞以及几种转化的3T3细胞的完整细胞膜中脂质酰基链的固有柔韧性。在正常和转化的小鼠成纤维细胞中未检测到脂质固有柔韧性的显著差异。这些结果与早期对鸡胚成纤维细胞获得的类似结果进行了比较。对于所研究的细胞，正常和转化成纤维细胞膜糖蛋白迁移率的差异要么已被直接证明，要么是通过其他研究从不同的凝集性推断出来的。因此，未找到将这些细胞中糖蛋白迁移率和脂质运动相关联的依据。
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