Provost P J, Wolanski B S, Miller W J, Ittensohn O L, McAleer W J, Hilleman M R
Proc Soc Exp Biol Med. 1975 Feb;148(2):532-9. doi: 10.3181/00379727-148-38578.
CR326 human hepatitis A virus purified by isopycnic banding from infected marmoset sera was shown to consist of 27 nm spherical particles on electron microscopic examination. The particles were identified as hepatitis A virus by tests for infectivity and by specific neutralization of infectivity with convalescent human hepatitis A serum. Also, indentical 27 nm viruses in liver extracts gave specific reactions with hepatitis A antisera when tested by immune electron microscopy. The bouyant density of the virus in CsCl was 1.34 and it was heat (60 degrees), ether and acid stable but was destroyed by heat (100 degrees), formalin (1:4000) and ultraviolet irradiation. Electron microscopic studies of sections of infected marmoset liver showed intracytoplasmic virus particles, usually in vesicles. Presumptive findings for RNA, together with the intracytoplasmic location of the virus, indicated the virus to be of RNA-type. The attributes of the virus indicate it is closely related to the enterovirus family and not to hepatitis B virus.
通过等密度梯度离心法从感染的狨猴血清中纯化得到的CR326型人甲型肝炎病毒，经电子显微镜检查显示由27纳米的球形颗粒组成。通过感染性试验以及用人甲型肝炎恢复期血清特异性中和感染性，这些颗粒被鉴定为人甲型肝炎病毒。此外，当通过免疫电子显微镜检测时，肝脏提取物中相同的27纳米病毒与甲型肝炎抗血清发生特异性反应。该病毒在氯化铯中的浮力密度为1.34，对热（60摄氏度）、乙醚和酸稳定，但可被热（100摄氏度）、福尔马林（1:4000）和紫外线照射破坏。对感染狨猴肝脏切片的电子显微镜研究显示，病毒颗粒存在于细胞质内，通常在小泡中。关于RNA的初步研究结果，以及病毒在细胞质内的定位，表明该病毒为RNA型。该病毒的特性表明它与肠道病毒科密切相关，而与乙型肝炎病毒无关。
