Huang S N, Lorenz D, Gerety R J
Lab Invest. 1979 Jul;41(1):63-71.
Electron and immunoelectron microscopic studies were carried out on liver tissues from three marmosets, experimentally infected with hepatitis A virus and sacrificed during the acute phase of illness. Ultrastructurally, the liver cells demonstrated marked cisternal dilation of endoplasmic reticulum and vesicular transformation and contortion of endoplasmic reticulum profiles. Clusters of virus-like particles of 24 to 27 nm. in diameter, both "solid" and "empty" forms, were found in membrane-bound cytoplasmic vesicles. In one animal, the virus-like particles were significantly smaller, measuring 17 to 22 nm. in size, and almost all were solid forms embedded in an amorphous matrix. Clusters of virus-like particles were found in the bile canaliculi of liver cell cords and in lysosomal structures of monocytes or Kupffer cells in the hepatic sinusoids. The latter correlated with the immunofluorescent microscopic finding. Indirect immunoferritin staining was carried out on fresh and formalin-fixed liver tissues, using convalescent phase serum from patients recovered from hepatitis A virus infection as the primary antibody, and the ferritin-labeled rabbit anti-human IgG or ferritin-labeled staphylococcal protein A as the secondary antibody. Specific stainings were observed with the virus-like particles, indicating that the particles were probably antigenically related to hepatitis A virus. Our findings are in agreement with the immunofluorescent and immunoelectron microscopic studies reported by others and support the concept that hepatitis A virus is produced in the liver. The infection seems to produce cytopathic effect especially to the endoplasmic reticulum organelle of hepatocytes.
对三只实验感染甲型肝炎病毒并在疾病急性期处死的狨猴的肝脏组织进行了电子显微镜和免疫电子显微镜研究。在超微结构上，肝细胞显示出内质网明显的池扩张以及内质网轮廓的泡状转化和扭曲。在膜结合的细胞质泡中发现了直径为24至27纳米的类病毒颗粒簇，包括“实心”和“空心”形式。在一只动物中，类病毒颗粒明显较小，大小为17至22纳米，几乎所有都是嵌入无定形基质中的实心形式。在肝细胞索的胆小管以及肝血窦中单核细胞或库普弗细胞的溶酶体结构中发现了类病毒颗粒簇。后者与免疫荧光显微镜检查结果相关。使用甲型肝炎病毒感染康复患者的恢复期血清作为一抗，铁蛋白标记的兔抗人IgG或铁蛋白标记的葡萄球菌蛋白A作为二抗，对新鲜和福尔马林固定的肝脏组织进行间接免疫铁蛋白染色。在类病毒颗粒上观察到特异性染色，表明这些颗粒可能在抗原性上与甲型肝炎病毒相关。我们的发现与其他人报道的免疫荧光和免疫电子显微镜研究结果一致，并支持甲型肝炎病毒在肝脏中产生的概念。这种感染似乎对肝细胞的内质网细胞器产生细胞病变效应。
