Tennant B C, Mrosovsky N, McLean K, Cote P J, Korba B E, Engle R E, Gerin J L, Wright J, Michener G R, Uhl E
Department of Clinical Sciences, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Hepatology. 1991 Jun;13(6):1215-21.
During studies of seasonal obesity, a high frequency of hepatic neoplasms was observed in Richardson's ground squirrels. Of 12 Richardson's ground squirrels examined thoroughly, 7 had mild or moderate degrees of chronic portal hepatitis and 6 (50%) had hepatocellular carcinoma. Serological tests for hepadnavirus surface antigen, anti-core antibody and virion DNA that recognize the ground squirrel hepatitis virus of California ground squirrels (Spermophilus beecheyi) were uniformly negative. Southern blot analyses of EcoRI digests of liver cell DNA demonstrated 3.2 kb fragments that hybridized with a ground squirrel hepatitis virus-specific probe in nontumorous liver tissue from 6 of 10 ground squirrels and in hepatocellular carcinoma specimens from 2 of 5 squirrels indicating infection with a hepadnavirus related to ground squirrel hepatitis virus. Failure, however, to detect serum antibody to ground squirrel hepatitis core antigen suggested probable antigenic differences between the ground squirrel hepatitis virus of California ground squirrels and the putative Richardson's ground squirrel agent. Further studies are required to fully characterize the hepadnavirus of Richardson's ground squirrels and to determine its relationship to hepatocarcinogenesis in this species.
在对季节性肥胖的研究过程中，在理查森地松鼠身上观察到了高频率的肝脏肿瘤。在对12只经过全面检查的理查森地松鼠中，7只患有轻度或中度慢性门脉性肝炎，6只（50%）患有肝细胞癌。针对地松鼠肝炎病毒表面抗原、抗核心抗体以及病毒粒子DNA进行的血清学检测，这些检测是用于识别加利福尼亚地松鼠（Spermophilus beecheyi）的地松鼠肝炎病毒的，结果均为阴性。对肝细胞DNA的EcoRI酶切产物进行的Southern印迹分析显示，在10只地松鼠中的6只的非肿瘤性肝组织以及5只松鼠中的2只的肝细胞癌标本中，有与地松鼠肝炎病毒特异性探针杂交的3.2 kb片段，这表明感染了一种与地松鼠肝炎病毒相关的嗜肝DNA病毒。然而，未能检测到针对地松鼠肝炎核心抗原的血清抗体，这表明加利福尼亚地松鼠的地松鼠肝炎病毒与假定的理查森地松鼠病原体之间可能存在抗原差异。需要进一步研究以全面表征理查森地松鼠的嗜肝DNA病毒，并确定其与该物种肝癌发生的关系。
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