一种用于马尔尼菲青霉的高分辨率多位点微卫星分型（MLMT）系统。
A highly discriminatory multilocus microsatellite typing (MLMT) system for Penicillium marneffei.
作者信息
Fisher Matthew C, DE Hoog Sybren, Akom Nongnuch Vanittan
出版信息
Mol Ecol Notes. 2004 Sep;4(3):515-518. doi: 10.1111/j.1471-8286.2004.00710.x.
Abstract
For eukaryotic pathogens that are depauperate in genetic variation, multilocus microsatellite typing (MLMT) offers an accurate and reproducible method of characterizing genetic diversity; herein we describe the development of an MLMT system for the emerging pathogenic fungus Penicillium marneffei based on 23 microsatellite loci. Screening isolates held within the Centraalbureau voor Schimmelcultures culture collection demonstrate high levels of genetic diversity and 100% reproducibility. This MLMT system provides a powerful epidemiological tool to analyse the underlying parameters that are responsible for the emergence of P. marneffei in human HIV-positive populations.
摘要
对于遗传变异较少的真核病原体，多位点微卫星分型（MLMT）提供了一种准确且可重复的遗传多样性表征方法；在此，我们描述了基于23个微卫星位点的新型致病真菌马尔尼菲青霉MLMT系统的开发。对保藏在荷兰真菌生物多样性中心菌种保藏库中的菌株进行筛选，结果显示出高水平的遗传多样性和100%的可重复性。该MLMT系统提供了一个强大的流行病学工具，用于分析导致马尔尼菲青霉在人类HIV阳性人群中出现的潜在参数。
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